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Method for simultaneously measuring alpha-acids, beta-acids and iso-alpha-acids in hops

A technology of hops and detection wavelength, which is applied in the field of chemical analysis and detection, can solve the problems of repeated experimental operation and waste of time, and achieve the effect of simple operation.

Inactive Publication Date: 2012-10-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology allows for efficient separation of different types of chemicals from each other by separating them into six major forms: alpha acid (C6H2O), beta acids (C7H8NO2), gamma ray steroids (C5HC4) or anisoleic acids called cinnamates that are found naturally throughout beer production worldwide.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and efficiency of detecting various chemical components such as alpha-, beta-, and other acids found naturally within hops used for making beverages like whisky due to its unique taste profile. Current methods require multiple steps and consume significant amounts of space on equipment.

Method used

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  • Method for simultaneously measuring alpha-acids, beta-acids and iso-alpha-acids in hops
  • Method for simultaneously measuring alpha-acids, beta-acids and iso-alpha-acids in hops

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Accurately weigh hop extract samples, accurately weigh 0.1g, place in a 50mL beaker, dissolve with 40mL methanol, place in an ultrasonic water bath for 10-20min, transfer to a 100mL volumetric flask, dilute to volume with methanol, and mix thoroughly. Filter with a 0.45μm organic filter, store in a sample bottle, and inject for analysis.

[0020] Reverse-phase liquid chromatography analysis conditions:

[0021] Chromatographic column: EC 250 / 4 Nuclesoil 100-5C18

[0022] Elution conditions: mobile phase: organic phase B is acetonitrile; inorganic phase A contains phosphoric acid (85%) 0.1%, 0.2mM EDTANa 2 of aqueous solution. The present invention adopts the gradient elution method, and the elution program is: 0-30min, 0%B-60%B; 30-32min, 60%B-70%; 32-50min, 70%B-75%B; 50- 55min, 75%B-60%B.

[0023] The injection volume was 10 μL, the flow rate was 1.0 mL / min, and the column temperature was 30 °C.

[0024] Detection wavelength: the detection wavelength of iso-α-aci...

Embodiment 2

[0027] Weigh 5.0g of granular hop samples ground into powder, put them in a 250mL conical flask with a stopper, extract with 20mL of methanol and 80mL of diethyl ether, vibrate on a shaker at a constant temperature of 20°C for 30min, add 40mL of 0.1mol / L hydrochloric acid solution, and then Oscillate for 30 minutes, rest and stratify. Take 5mL of the upper ether layer, dilute it to 50mL with methanol, make it fully uniform, filter it with a 0.45μm organic filter membrane, store it in a sample bottle, and inject it for analysis.

[0028] Reverse-phase liquid chromatography analysis conditions:

[0029] Chromatographic column: EC 250 / 4 Nuclesoil 100-5C18

[0030] Elution conditions: mobile phase: organic phase B is acetonitrile; inorganic phase A contains phosphoric acid (85%) 0.1%, 0.2mM EDTANa 2 of aqueous solution. The present invention adopts the gradient elution method, and the elution program is: 0-30min, 0%B-60%B; 30-32min, 60%B-70%; 32-50min, 70%B-75%B; 50- 55min, 75...

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Abstract

The invention discloses a method for simultaneously measuring alpha-acids, beta-acids and iso-alpha-acids in hops by reverse-phase high-performance chromatography. By the aid of the method, nine substances including three types of the alpha-acids, three types of the beta-acids and three types of the iso-alpha-acids can be simultaneously separated quickly and effectively. The method is characterized in that chromatographic conditions include that a chromatographic column is an EC 250/4Nucleosoil 100-5C18 chromatographic column, a flowing phase A is aqueous solution [containing 0.1% of phosphoric acids and 0.2mM of EDTANa2 (ethylene diamine tetraacetic acid disodium)], and a flowing phase B is acetonitrile. The flowing speed is 1.0mL/min, dual-wavelength detection UV (ultraviolet) waves have wavelengths of 270nm and 315nm, the temperature of the column is 30 DEG C, and the volume of an injected sample is 10 microliters. The method is used for monitoring change relation among the alpha-acids, the beta-acids and the iso-alpha-acids in the hops in a production process for heterogenous particles and heterogenous extract of the hops, and provides a theoretical reference for production technology. In addition, the method also can be used for researching dynamic variation of the alpha-acids and the iso-alpha-acids of the hops in a wort boiling process. Besides, the method has the advantages of good stability and repeatability, simplicity in operation, accuracy and reliability.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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