Purinamine compound and preparation method thereof
A compound, purine amine technology, applied in the field of pharmaceutical chemical synthesis, can solve the problems of high cancer recurrence rate, toxicity, unsatisfactory treatment effect, etc., and achieve the effect of inhibiting tumor growth
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Embodiment 1
[0023] The preparation of embodiment 1 intermediate [1,2,4] triazol [1,5-c] pyrimidin-5-amine
[0024]
[0025] Dissolve 100g of 5-chloro[1,2,4]-triazol[1,5-c]pyrimidine in 200ml of saturated ammonia methanol solution, stir at room temperature for 24 hours, remove the solvent by rotary evaporation, and add a small amount of 43.7g of solid [1,2,4]triazol[1,5-c]pyrimidin-5-amine was generated by filtration, the yield was 50%, MS: [M+H] + 136.1.
Embodiment 2
[0026] Example 2 Preparation of intermediate N-(2-chloro-9-methyl-9H-purin-6-yl)-[1,2,4]triazol[1,5-c]pyrimidin-5-amine
[0027]
[0028] At room temperature, 10g of 2,6-dichloro-9-methyl-9H-purine was dissolved in 100ml of absolute ethanol, and 7g of diisopropylethylamine and 7.32g of diisopropylethylamine prepared in Example 1 were added to this solution [1,2,4]triazol[1,5-c]pyrimidin-5-amine, reflux overnight, remove the solvent by rotary evaporation, add cold water to the residue to form a solid, filter, and dry in vacuo to obtain N-(2- Preparation of chloro-9-methyl-9H-purin-6-yl)-[1,2,4]triazol[1,5-c]pyrimidin-5-amine 12g, 81% yield, MS, m / Z: [M+H] + 302.7.
Embodiment 3
[0029] Example 3 Intermediate N-(2-chloro-9-methyl-9H-purin-6-yl)-N-methyl-[1,2,4]triazol[1,5-c]pyrimidine-5 - Preparation of amines
[0030]
[0031] At room temperature, 5 g of N-(2-chloro-9-methyl-9H-purin-6-yl)-[1,2,4]triazol[1,5-c]pyrimidine- 5-Amine was dissolved in 50 ml of DMF, and 2.36 g of potassium carbonate was added to this solution. The reaction solution was cooled to 0°C, 3.53 g of iodomethane was added, and stirred at room temperature for 12 hours. The reaction solution was slowly poured into stirred water, and the resulting solid was filtered and dried to obtain N-(2-chloro-9-methyl-9H-purin-6-yl)-N-methyl-[1,2,4 ] Triazol[1,5-c]pyrimidin-5-amine 5.18g, yield 99%. MS: [M+H] + 316.7.
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