Method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules

A technology of stilbene glucoside and salvianolic acid, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of improving detection efficiency, easy popularization and mastering, and no evaporation

Active Publication Date: 2015-07-08
SHIJIAZHUANG ZANGNUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the simultaneous determination of two components using the same mobile phase.

Method used

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  • Method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules
  • Method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules
  • Method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules

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Embodiment 1

[0034] (1) Chromatographic conditions and system suitability test: Octadecylsilane bonded silica gel is used as filler; methanol-acetonitrile-1.5% formic acid aqueous solution with volume ratio of 23~24:8~9:65~67 is used as mobile phase ;The detection wavelength is 300nm; the number of theoretical plates calculated based on the peak of salvianolic acid B should not be less than 2000;

[0035] (2) Preparation of reference substance solution Take an appropriate amount of stilbene glucoside and salvianolic acid B reference substance, weigh them accurately, add methanol to make a stock solution, then take the stock solution and add 70% methanol to make each 1ml containing 20 μg stilbene glycoside, salvianolic acid B A solution of 30 μg of phenolic acid B is available;

[0036] (3) Preparation of the test solution Take an appropriate amount of the contents of Guidan Seabuckthorn Capsules, grind it finely, take about 0.1g, weigh it accurately, put it in a 25ml volumetric flask, add ...

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Abstract

The invention relates to a method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules. The method for simultaneously quantifying stilbene glucoside and salvianolic acid B in Guidansaji capsules is characterized in that common isocratic elution is adopted, methanol-acetonitrile-1.5 percent formic acid water solution in the volume ratio of 23-24:8-9:65-67 is used as a mobile phase; the contents of stilbene glucoside and salvianolic acid B in a sample are simultaneously determined at the wavelength of 300+ / -2nm so that two traditional Chinese medicine active components with completely different properties can adopt the same mobile phase and are quantitatively determined once; and the quantitative chromatogram of the two components shows that peak separation is good, the wave peak retention time of tilbene glucoside is about 5 minutes, and the wave peak retention time of salvianolic acid B is about 15 minutes. The method has no extraction, no concentration and no evaporation, is simple, convenient, accurate and repeatable, is easy to popularize and master, and effectively improves the detection efficiency and reduces the detection cost and environment pollution.

Description

technical field [0001] The invention relates to a method for simultaneously quantifying stilbene glycosides and salvianolic acid B in Guidan seabuckthorn capsules. Background technique [0002] Guidan Seabuckthorn Capsule is a health food approved by the State Food and Drug Administration. The approval number is: Guoshi Jianzi 620110772. Its health care function is laxative and laxative, removing chloasma. The composition of each 1000 capsules prescription is as follows: [0003] Polygonum multiflorum 500g Angelica 500g Bitter almond 400g [0004] Seabuckthorn 250g Salvia 200g [0005] Preparation process: Take 100g of the five herbs in the prescription, dry it at 60°C, crush it, pass it through a 80-mesh sieve, and use 5kGy of 60 Co irradiated and sterilized for later use; add 8 times the amount of 75% ethanol to the remaining Danshenshen seabuckthorn, reflux extraction twice, 1.5 hours each time, filter, combine filtrates, recover ethanol under reduced pressure, and co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/26
Inventor 韩桂茹王智森高飞
Owner SHIJIAZHUANG ZANGNUO BIOTECH
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