Endophytic fungus with preventive and treatment effects on soft rot of dendrobiums

An endophytic fungus and soft rot technology, applied in the field of bioengineering, can solve the problems of low disease control efficiency, no harvest of cultivated crops, and lack of standardized operation technology.

Active Publication Date: 2012-11-14
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the disease is serious, soft rot can cause large-scale failure of cultivated crops, which seriously restricts the development of Dendrobium industry
At present, there are mainly two ways to prevent and treat the soft rot of Dendrobium plants: one is through cultivation management, such as controlling the humidity of the seedbed, ventilation, disinfection of water sources, and sunlight exposure treatment of the cultivation substrate, etc., which can play a role in the prevention and control of the disease. However, this kind of method is labor-intensive and time-consuming, the operation technology is not standardized, the controllability is poor, and the efficiency of disease control is not high; However, it is easy to cause drug resistance of pathogenic bacteria, and the extensive use of pesticides has a great effect on environmental pollution and ecological balance damage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Biocontrol of Dendrobium nobile soft rot by Dhs96

[0014] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were collected after activation and cultured, and prepared into 5×10 5 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated into the roots of the Dendrobium nobile seedlings when the Dendrobium nobile seedlings are planted. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium nobile seedlings 3 days and 30 days after inoculating...

Embodiment 2

[0016] Biological control of soft rot in Dendrobium officinale seedlings by Dhs96

[0017] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were activated and cultured to prepare 1×10 5 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated into the roots of the Dendrobium officinale seedlings when the Dendrobium officinale seedlings are planted. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium officinale seedlings 3 days and 30 days after...

Embodiment 3

[0019] Biological control of Dhs96 on soft rot of Dendrobium chrysanthemum seedlings

[0020] The endophytic fungus Dhs96 was cultured by PDA liquid fermentation and mixed evenly with wheat bran solid matrix to make solid inoculant. The spores of Dendrobium soft rot pathogen Pythium ultimum (Pythium ultimum) were collected after activation and cultured, and prepared into 5×10 4 / ml of spore suspension, mixed evenly with wheat bran solid matrix to make solid inoculant. The bark is used as the cultivation substrate, and the Dhs96 solid inoculant is inoculated to the roots of the Dendrobium chrysotox seedlings when planting the Dendrobium chrysotox seedlings. The potted seedlings were placed in a solar greenhouse, and the temperature was maintained at 20±2°C. Spray water after planting to facilitate the growth of endophytic fungi. After that, spray water every three days. The solid inoculants of pathogenic bacteria were sprinkled around the Dendrobium drumstick seedlings 3 da...

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Abstract

The invention discloses an endophytic fungus with preventive and treatment effects on soft rot of dendrobiums. The fungus can be subjected to field planting at the root of dendrobiums, and is used for seedling raising or cultivation of dendrobiums. The endophytic fungus with preventive and treatment effects on soft rot of dendrobiums can effectively prevent the soft rod of dendrobiums from happening.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to two endophytic fungal strains with biological control effects on soft rot of Dendrobium plants. Using the two strains of fungi in the seedling raising or cultivation of the Dendrobium can effectively prevent the soft rot of the Dendrobium. Background technique [0002] There are many traditional and precious Chinese medicinal materials in Orchidaceae, such as Dendrobium officinale and Dendrobium nobile. According to the records of the 2005 edition of "Chinese Pharmacopoeia", Dendrobium nobile LindL., Dendrobium officinale (D.candidum Wall, ex Lind1.) or Dendrobium nobile (D. fimbriatum Hook, var. The fresh or dried stems of the species can be used as a traditional Chinese medicine Dendrobium, which has the effects of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, and can also relieve throat and improve eyesight, and regulate human immun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01G13/00C12R1/845
Inventor 郭顺星李向东陈晓梅王卉
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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