Method for breeding freshly eaten corn with eye health-care effect

A health-care effect, fresh-eating technology, applied in the directions of botanical equipment and methods, measuring devices, instruments, etc., can solve the problems of decreasing zeaxanthin content, unfocused breeding of zeaxanthin, etc. Increase the efficiency of early detection and prevent presbyopia

Active Publication Date: 2012-11-14
JINHUA ACAD OF AGRI SCI
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Problems solved by technology

[0009] The purpose of the present invention is to solve the defects that the zeaxanthin content in most fresh corns is less, and the corn breeding method does not focus on the selection of zeaxanthin, which leads to a downward trend in the zeaxanthin content of most corns at present. , providing a method for breeding fresh corn with eye health effects

Method used

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  • Method for breeding freshly eaten corn with eye health-care effect
  • Method for breeding freshly eaten corn with eye health-care effect

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Embodiment 1

[0039] Breeding method of functional fresh corn with zeaxanthin content up to 9.3mg / kg:

[0040]Using the sweet corn variety "Huazhen" as the starting material, after three generations of self-breeding, the dry seeds of the excellent inbred line "Hua 36-9" with a water content of 13.5% were irradiated with cobalt-60 gamma rays , the irradiation dose is the conventional dose of 200Gy. Live-direct mutagenesis of contemporary seeds, and mixed harvest of seeds of mutagenesis first-generation plants. Mixed cultivation of the second-generation population material. About 21 days after pollination, peel off the whole grain and cut it longitudinally along the middle seam of the grain with a knife, and select the material whose endosperm is all yellow or light yellow. The saponified matter was extracted, and the total carotenoid content in fresh corn kernels of all materials was detected by UV-Vis spectrophotometry. The materials with a total carotenoid content of more than 10 mg / kg ...

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Abstract

The invention relates to a method for breeding freshly eaten corn with an eye health-care effect and aims to overcome the defect that the rubixanthine content of most of corn is reduced gradually because the corn rubixanthine in most of the current freshly eaten corn is low and the breeding method of the corn is not emphasized on breeding the corn rubixanthine. The method comprises the following steps of taking an inbred line of the freshly eaten corn as an initial material, and selecting the corn freshly eaten corn which can stably express and has over 8mg / kg of rubixanthine by ultraviolet-visible spectrophotometry and high-performance liquid chromatography, or taking the corn material rich in corn rubixanthine gene as a donor, and performing hybridization, backcross or somaclonal variation to obtain the inbred line or a hybrid variety of the corn rich in the corn rubixanthine finally.

Description

technical field [0001] The invention relates to a breeding method of corn, in particular to a breeding method of fresh corn with eye health care effect. Background technique [0002] With the aging of society and the continuous aggravation of people's excessive use of eyes, vision loss, macular degeneration, cataract, diabetic retinopathy, floaters, glaucoma and other common eye diseases in middle-aged and elderly people will gradually become common and younger. The root of the material basis lies in the long-term lack of "eye gold" - zeaxanthin, which cannot be synthesized by the human body and must come from the diet. [0003] Zeaxanthin (Zeaxanthin, 3,3′-dihydroxy-β-carotene) is an isoprenoid unsaturated compound, which belongs to a kind of carotenoid, and its molecular formula is C40H56O2. It is often associated with β-carotene, leaf Flavin, etc. coexist and widely exist in various tissues of the human body, but its distribution in the eye is the most prominent and char...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/33G01N30/02A01H1/02
Inventor 袁名安吴殿星刘新华刘莉舒小丽曹春信徐秀红
Owner JINHUA ACAD OF AGRI SCI
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