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Demulsification method by soybean grease microbe extraction through aqueous enzymatic method

A technology of soybean oil and microorganism, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc. It can solve the problems of low demulsification rate and environmental pollution

Active Publication Date: 2014-03-26
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, foreign scholars mostly use organic solvents to directly extract the oil in the emulsion in order to investigate the effect of releasing the oil from the oil plant cells after enzymatic hydrolysis, but no further research on the technical level has been reported, and, now Some demulsification technologies have problems such as low demulsification rate and environmental pollution

Method used

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  • Demulsification method by soybean grease microbe extraction through aqueous enzymatic method
  • Demulsification method by soybean grease microbe extraction through aqueous enzymatic method
  • Demulsification method by soybean grease microbe extraction through aqueous enzymatic method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening test for optimal compound ratio of mixed demulsifiers

[0028] 1 Materials and methods

[0029] 1.1 Test raw materials

[0030] Dehulled Soybean Flakes Harbin Jiusan Oil Group protex-6L alkaline endoprotease Danish novo company Bacillus mohewei Key Laboratory of Northeast Agricultural University Bacillus subtilis Key Laboratory of Northeast Agricultural University Nocardia sludge Key Laboratory of Northeast Agricultural University MMSM inorganic salt liquid medium NH 4 NO 3 4.0g, K 2 HPO 4 4.0g, KH 2 PO 4 6.0g, MgSO 4 ·H 2 O 0.2g, trace element solution 1mL, liquid paraffin 4% (v / v), yeast extract 1.0g, glucose 10.0g, deionized water 1000mL. Sterilize at 121°C for 15 minutes.

[0031] 1.2 Test instruments and equipment

[0032] instrument Manufacturer CX-500 Ultrasonic Cleaning Machine Beijing Second Medical Equipment Factory Autoclave Tianjin Test Instr...

Embodiment 2

[0061] Embodiment 2: the screening experiment of the optimal parameter of the fermentation of mixed demulsifier demulsification

[0062] Based on the optimal compound ratio of the compound biological demulsifier determined in Example 1, the fermentation test of the single-factor compound biological demulsifier was carried out to determine the fermentation process parameters (shaking table speed, inoculum size, culture temperature, culture time) range. Taking the demulsification rate as an investigation index, a response surface design experiment with 4 factors and 5 levels was carried out.

[0063] 1 Materials and methods

[0064] 1.1 Materials and reagents

[0065] Dehulled Soybean Flakes Harbin Jiusan Oil Group protex-6L alkaline endoprotease Danish novo company Bacillus mohewei Key Laboratory of Northeast Agricultural University Bacillus subtilis Key Laboratory of Northeast Agricultural University Nocardia sludge Key Laboratory of N...

Embodiment 3

[0095] Example 3 The screening experiment of the optimal parameters of mixed demulsifier demulsification

[0096] 1.1 Materials and reagents

[0097] Dehulled Soybean Flakes Harbin Jiusan Oil Group protex-6L alkaline endoprotease Danish novo company Bacillus mohewei Key Laboratory of Northeast Agricultural University Bacillus subtilis Key Laboratory of Northeast Agricultural University Nocardia sludge Key Laboratory of Northeast Agricultural University MMSM inorganic salt liquid medium NH 4 NO 3 4.0g, K 2 HPO 4 4.0g, KH 2 PO 4 6.0g, MgSO 4 ·H 2 O 0.2g, trace element solution 1mL, liquid paraffin 4% (v / v), yeast extract 1.0g, glucose 10.0g, deionized water 1000mL. Sterilize at 121°C for 15 minutes.

[0098] 1.2 Main instruments and equipment

[0099] pHS-25 acidity meter Shanghai Weiye Instrument Factory electronic analytical balance Merlot-Toledo Instruments (Shanghai) Co., Ltd. centr...

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Abstract

The invention relates to a demulsification method by soybean grease microbe extraction through an aqueous enzymatic method, which belongs to the plant grease extraction technology. The method comprises the following steps: 1) crushing soybean to obtain a puffed material by using an extruding puffed pretreatment, mixing the puffed material and water to obtain a mixed liquor, adding alkaline protease in the mixed liquor for enzymatic hydrolysis, centrifuging after enzymatic hydrolysis to obtain free oil, an emulsion, a hydrolysate and residues; 2) fermenting the mixed demulsifying bacteria with different complex formulation proportion through a MMSM inorganic salt liquid medium to obtain a mixed demulsifying bacteria whole liquid culture medium; 3) adjusting the pH value to the emulsion obtained in the step 1) to neutrality, fully and uniformly mixing with the mixed demulsifying bacteria whole liquid culture medium, and standing and performing constant temperature demulsification, centrifuging after demulsification to obtain soybean oil. The method of the invention has the advantages of simple technology device, low cost, easy degradation of waste liquid, low toxicity and no pollution generation, and the mixed demulsifying bacteria whole liquid culture medium has good thermostability and repeated usage.

Description

technical field [0001] The invention belongs to vegetable oil extraction and processing technology, and mainly relates to a method for microbial demulsification of soybean oil extracted by aqueous enzymatic method. Background technique [0002] Demulsification is a very critical step in the method of aqueous enzymatic extraction of soybean oil. The more thorough the demulsification, the higher the recovery rate of oil. The quality of demulsification directly affects the economic value of the whole process. [0003] Demulsification methods are divided into chemical demulsification, physical demulsification and biological demulsification. The chemical demulsification method is to add a chemical demulsifier; centrifugation, changing the temperature, applying an electrostatic field, and using a coalescing agent are all physical methods; the biological demulsification method refers to the use of microbial cells themselves or metabolites of their metabolic processes to achieve em...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B3/00C11B1/00
Inventor 江连洲李杨齐宝坤于鹏王中江王胜男
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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