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Biological method for preparing gamma-aminobutyric acid

A technology of aminobutyric acid and aminobutyric acid clear solution, which is applied in the field of preparation of γ-aminobutyric acid, can solve the problems of cumbersome separation and purification process, restriction of industrial production, and complex components of fermentation broth, so as to improve product purity, low cost, Post-processing simple effects

Active Publication Date: 2013-11-20
南通励成生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the existing methods, γ-aminobutyric acid is mostly produced in the fermentation broth. Due to the complex components of the fermentation broth, the downstream separation and purification process is cumbersome, and the concentration of the target product is also low. These factors limit domestic industrial production, and the preparation of high-purity γ-aminobutyric acid is rarely reported

Method used

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Embodiment 1

[0032] 1. Bacteria culture

[0033] After the lactic acid bacteria strains are activated, the first-level seed culture, the second-level seed culture, and finally enter the fermenter culture, the inoculation amount is 3% (V / V, seed liquid / fermentation liquid), 30 ℃ static culture for 16 hours, the fermentation process The pH is controlled by adding lye, so that the pH of the fermentation broth is maintained in the range of 4.5-7.0, and the bacteria are collected by filtration through a 0.6 μm ceramic membrane.

[0034] Seed medium raw materials in g / L: glucose 10, yeast extract 5, peptone 5, sodium acetate 0.5, disodium hydrogen phosphate (or dipotassium hydrogen phosphate) 1, magnesium sulfate 1, manganese sulfate 1, PPE polyether defoaming Agent 0.1 (but not limited to this defoamer, any defoamer that can be used in the fermentation industry can be used).

[0035] Fermentation medium formula in g / L: glucose 10, yeast extract 5, peptone 5, sodium acetate 0.5, sodium glutamat...

Embodiment 2

[0053] A method for preparing gamma-aminobutyric acid by a biological method, comprising the following steps:

[0054] (1) Cell preparation:

[0055] Add sodium glutamate to the medium, and add 1-30g / L (for example, 1 g / L, 15 g / L, 30 g / L) as an inducer to induce enzyme production and insert it into the producing cells The strain of glutamic acid decarboxylase is cultivated; the culture conditions are: culture temperature 25-35°C (for example, 25°C, 30°C, 35°C), pH control is carried out by adding lye during the fermentation process, so that the pH of the fermentation broth is maintained at Within the range of 4.5-7.0, the culture time is 8 to 24 hours (for example, 8h, 16h, 24h); the bacterial liquid is obtained after the culture;

[0056] (2) Collection of bacterial cells by ceramic membrane filtration: filter the bacterial liquid obtained in step (1) through a ceramic membrane to collect bacterial cells with a membrane pore size of 0.05-1 μm (for example, 0.05 μm, 0.5 μm, 1...

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Abstract

The present invention discloses a biological method for preparing gamma-aminobutyric acid, including the steps of: preparing bacteria, producing gamma-aminobutyric acid, decoloring, conducting electrodialysis, preparing gamma-aminobutyric acid powder products or gamma-aminobutyric acid crystal products and the like. The present invention utilizes the free lactic acid bacterium cells for catalyzed synthesis of gamma-aminobutyric acid, and helps to improve the production technology, improve the gamma-aminobutyric acid yield, and reduce the cost, and also establishes the method for preparation of high-purity gamma-aminobutyric acid. The reaction system of the present method does not include carbon sources, nitrogen sources and other culture components, and the reaction liquid components are simple relative to fermentation liquid. The biological method can greatly improve the purity of the product, and has the advantages of simple post-treatment, short production cycle, little environmental pollution, low cost, etc.

Description

technical field [0001] The invention relates to a method for preparing gamma-aminobutyric acid. Background technique [0002] Gamma-aminobutyric acid (GABA), also known as aminobutyric acid, is a naturally occurring non-protein amino acid that is widely distributed in prokaryotes and eukaryotes. γ-aminobutyric acid is an inhibitory transmitter substance of the mammalian central nervous system. It has important physiological functions such as calming the nerves, promoting sleep, lowering blood pressure, strengthening the brain and improving intelligence, delaying aging, strengthening the liver and kidney. [0003] So far, the preparation methods of γ-aminobutyric acid mainly include chemical synthesis and biological methods. A common chemical synthesis method is to prepare γ-aminobutyric acid by hydrolyzing calcium hydroxide and ammonium bicarbonate with pyrrolidone as a raw material. The cost of chemical synthesis is high, the yield is low, and dangerous solvents are used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C07C229/08C07C227/40C12R1/225
Inventor 沈宇峰帅玉英顾钦青吴晓花李佶松
Owner 南通励成生物技术有限公司
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