Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for measuring breast cancer HER2 gene expression and kit thereof

A technology of gene expression and kits, applied in the fields of biotechnology and medicine, can solve the problems of being unsuitable for routine clinical testing, short storage time of probes, and high cost, and achieve high accuracy, good sensitivity, and pollution-reducing effects

Active Publication Date: 2014-11-19
SHENZHEN INST OF ADVANCED TECH
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quantum dot detection method used in the prior art generally uses quantum dots to be directly combined with antibodies to make probes to achieve simultaneous labeling of multiple antigens. This method is costly and the probes have a short storage time. Suitable for routine clinical testing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring breast cancer HER2 gene expression and kit thereof
  • Method for measuring breast cancer HER2 gene expression and kit thereof
  • Method for measuring breast cancer HER2 gene expression and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] One, the described test kit includes:

[0046] (1) Antibody dilution buffer: a mixed solution of phosphoric acid and NaCl, wherein the concentrations of phosphoric acid and NaCl are both 0.01mol / L;

[0047] (2) Incubation buffer: a mixed solution prepared by phosphoric acid, NaCl, and fetal bovine serum, the concentration of phosphoric acid and NaCl is 0.01mol / L, and the concentration of fetal bovine serum is 5%;

[0048] (3) Solution of bipyridyl ruthenium-labeled HER2 monoclonal antibody: the concentration is 1×10 -6 mol / L;

[0049] (4) Aqueous solution of ZnSe / ZnS core / shell quantum dots: the concentration is 2.0×10 -6 mol / L;

[0050] Two, the preparation of described kit:

[0051] 1. Preparation of bipyridyl ruthenium-labeled HER2 monoclonal antibody

[0052] Dissolve 50 μg of dry powder of HER2 monoclonal antibody in 500 μL of phosphate buffer solution (pH=8.0), add 1 mg of bipyridyl ruthenium solid powder activated by NHS (N-Hydroxysuccinimide, N-hydroxysuccini...

Embodiment 2

[0062] One, the described test kit includes:

[0063] (1) Antibody dilution buffer: a mixed solution of phosphoric acid and NaCl, wherein the concentrations of phosphoric acid and NaCl are both 0.02mol / L;

[0064] (2) Incubation buffer: a mixed solution prepared by phosphoric acid, NaCl, and fetal bovine serum, the concentration of phosphoric acid and NaCl is 0.02mol / L, and the concentration of fetal bovine serum is 10%;

[0065] (3) HER2 monoclonal antibody solution labeled with bipyridyl ruthenium: the concentration is 1×10 -6 mol / L;

[0066] (4) ZnSe / ZnS core / shell quantum dot aqueous solution: the concentration is 1.0×10 -6 mol / L;

[0067] Second, the application of the kit:

[0068] Detection of human breast cancer cells and human embryonic kidney cells with a kit from Yizhong

[0069] (1) Dilute the solution of the HER2 monoclonal antibody labeled with bipyridyl ruthenium with antibody dilution buffer so that the concentration of the HER2 monoclonal antibody labeled...

Embodiment 3

[0075] One, the described test kit includes:

[0076] (1) Antibody dilution buffer: a mixed solution of phosphoric acid and NaCl, wherein the concentrations of phosphoric acid and NaCl are both 0.03mol / L;

[0077] (2) Incubation buffer: a mixed solution prepared by phosphoric acid, NaCl, and fetal bovine serum, the concentration of phosphoric acid and NaCl is 0.03mol / L, and the concentration of fetal bovine serum is 20%;

[0078] (3) HER2 monoclonal antibody solution labeled with bipyridyl ruthenium: the concentration is 2×10 -6 mol / L;

[0079] (4) ZnSe / ZnS core / shell quantum dot aqueous solution: the concentration is 2.0×10 -6 mol / L;

[0080] Second, the application of the kit:

[0081] BT474 cells with different densities were detected with the kit in Yizhong

[0082] (1) Dilute the HER2 monoclonal antibody solution labeled with bipyridyl ruthenium with antibody dilution buffer, so that the concentration of the HER2 monoclonal antibody labeled with bipyridyl ruthenium i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quantum dotaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for measuring breast cancer HER2 gene expression and a kit thereof. The kit disclosed herein comprises (1) an antibody dilution buffer, (2) an incubation buffer, (3) a solution of bipyridine ruthenium labelled HER2 monoclonal antibody, and (4) an aqueous solution of Cd-free quantum dots, wherein the antibody dilution buffer is a phosphoric acid-NaCl buffer prepared by mixing phosphoric acid and NaCl, and the incubation buffer is prepared by mixing the antibody dilution buffer with fetal calf serum. Experiments prove that the method disclosed herein has the advantages of simple operation, high sensitivity and high accuracy.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and relates to a method for detecting the expression level of breast cancer HER2 gene and a kit thereof. Background technique [0002] Breast cancer is one of the most common malignant tumors in women and the "number one killer" that threatens women's health. With the change of people's lifestyle, the incidence of breast cancer is also increasing year by year. Every year, about 1.4 million women worldwide suffer from breast cancer, and 400,000 women die from breast cancer. According to the American Cancer Statistical Expectation data released by the American Cancer Society in 2012, breast cancer ranks first in the incidence of female malignancies, accounting for 29% of all new female malignancies. In my country, the incidence of breast cancer has been increasing year by year in recent years. From 1982 to 2001, the incidence of breast cancer in women increased by 91%. Recent statisti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577
Inventor 蔡林涛盛宗海胡德红龚萍张鹏飞高笃阳
Owner SHENZHEN INST OF ADVANCED TECH