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Method for preparing lactic acid by soapberry seed shell

A technology of Sapindus chinensis and lactic acid, applied in fermentation and other directions, can solve the problems of high cost and large food consumption, and achieve the effect of small investment, reduced consumption and large amount of resources

Inactive Publication Date: 2014-05-07
BEIJING FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main raw material used in traditional lactic acid fermentation is starch, which is expensive, and large-scale industrial production will consume a lot of food

Method used

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  • Method for preparing lactic acid by soapberry seed shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Put 1 kg of sapindus seed husks crushed into 40 meshes in 20 L of alkali-alcohol solution (20% 1M NaOH solution, 80% ethanol), treat at 80°C for 3 hours, then filter, wash the residue with hot water until neutral, and then carry out synchronous saccharification Fermentation converts lactic acid. In 1 liter of fermentation system, the addition amount of alkali-alcohol-treated Sapindus seed husk is 130 g, the addition amount of cellulase is 15 FPU / g cellulose, and the addition amount of lactic acid bacteria strain is 3 g (dry matter). Carry out synchronous saccharification and fermentation for 120 hours, the reaction temperature is 45° C., and the pH value is 4.8. The composition of the fermentation medium is KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l, beef extract 5g / l, solid CaCO 3 20g / l. The yield of lactic acid is 99.5%.

Embodiment 2

[0022] Put 1 kg of sapindus seed husks crushed into 40 meshes in 20 L of alkali-alcohol solution (20% 1M NaOH solution, 80% ethanol), treat at 80°C for 3 hours, then filter, wash the residue with hot water until neutral, and then carry out synchronous saccharification Fermentation converts lactic acid. In 1 liter of fermentation system, the amount of sapindus seed husks treated with alkali alcohol is 150 g, the amount of cellulase is 15 FPU / g cellulose, and the amount of lactic acid bacteria is 2 g (in terms of dry matter). Carry out synchronous saccharification and fermentation for 96 hours, the reaction temperature is 48°C, and the pH value is 4.8. The composition of the fermentation medium is KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l, beef extract 5g / l, solid CaCO 3 20g / l. The yield of lactic acid was 92.8%.

Embodiment 3

[0024] Put 1 kg of sapindus seed husks crushed into 40 meshes in 20 L of alkali-alcohol solution (20% 1M NaOH solution, 80% ethanol), treat at 80°C for 3 hours, then filter, wash the residue with hot water until neutral, and then carry out synchronous saccharification Fermentation converts lactic acid. In 1 liter of fermentation system, the amount of sapindus seed husks treated with alkali alcohol is 120 g, the amount of cellulase is 15 FPU / g cellulose, and the amount of lactic acid bacteria is 4 g (in terms of dry matter). Carry out synchronous saccharification and fermentation for 120 hours, the reaction temperature is 46°C, and the pH value is 4.8. The composition of the fermentation medium is KH 2 PO 4 0.5g / l, MgSO 4 ·7H 2 O 0.5g / l, NaCl 0.1g / l, beef extract 5g / l, solid CaCO 3 20g / l. The yield of lactic acid was 98.0%.

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Abstract

The invention relates to a method for preparing lactic acid by soapberry seed shells, which comprises the following steps: treating soapberry seed shells in an alkali alcoholic solution, filtering the solution, washing residues of the soapberry seed shells, and then performing simultaneous saccharification and fermentation to convert into lactic acid. The invention adopts soapberry seed shells which is abundant in resources and large in unit weight, and comprises a step of alkali alcohol pretreatment for separating lignin and hemicellulose; the soapberry seed shell resource is made full use of; and the production efficiency of lactic acid is improved.

Description

technical field [0001] The invention relates to a method for preparing lactic acid, in particular to a method for preparing lactic acid from sapindus seed husks, belonging to the field of biomass chemistry and chemical engineering. Background technique [0002] Sapindus Mukorossi Gaertn, belonging to Sapindaceae (Sapindaceae), is native to the south of the Yangtze River Basin, Indochina Peninsula, India and Japan. Sapindus can be used for greening, and the fruit stone can be used to make natural handicrafts. Its roots, stems, and fruits can be used as medicine. Its functions and indications: clearing away heat, eliminating phlegm, eliminating accumulation, killing insects, etc., for sore throat, swelling and pain, cough and asthma , food stagnation, leucorrhea, malnutrition, sores, swelling, hepatitis B, etc. have better results. Sapindus arils produce an average of 20kg of fruit per plant during the full fruit period, and its arils are rich in saponins, mainly terpenoid sa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56
Inventor 蒋建新卜令习陈清英冯月唐勇朱莉伟
Owner BEIJING FORESTRY UNIVERSITY
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