Method for predicting release amount of phenol in mainstream smoke of flue-cured tobaccos
A technology of mainstream smoke, prediction method, applied in the field of tobacco
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[0077] (1) Pretreatment of samples to be tested
[0078] The pre-treatment of the raw flue-cured tobacco leaves to be tested involves three steps: manual tearing, stem removal, and shredding. The processed tobacco slices (silk) are immediately sealed, labeled, and stored uniformly without confusion. Before and after rehydration and shredding of different smoke samples, the equipment is carefully cleaned to ensure that the smoke samples are not skewered.
[0079] (2) Select 1 sample of uncured tobacco leaf to determine 8 kinds of chemical components: linoleic acid, total sugar, total nitrogen, chlorogenic acid, malonic acid, total polyphenols, tobacco nicotine, volatile acid, by microwave-assisted Derivatization – Determination of non-volatile organic acids (linoleic acid, malonic acid) by gas chromatography; determination of water-soluble sugars (total sugars) by YC / T159-2002 continuous flow method; determination by YC / T161-2002 continuous flow method Total nitrogen; Determi...
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