A High Performance Liquid Chromatography Evaporative Light Scattering Detection Method for the Simultaneous Separation and Detection of Thirteen Sugars and Sugar Alcohols
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A high-performance liquid chromatography and evaporative light scattering technology, which is used in material separation, measurement devices, analysis materials, etc., to achieve the effects of simple operation, low detection limit, rapid and accurate characterization
Active Publication Date: 2017-04-12
苏州市产品质量监督检验院 +1
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At present, there is no report on the simultaneous separation and detection of the above thirteen kinds of monosaccharides, disaccharides, functional oligosaccharides, and functional sugar alcohols using HPLC-ELSD method
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Embodiment 1
[0022] Dilute the concentrations of various single standard solutions of 10mg / ml to 5mg / ml, 3mg / ml, 2mg / ml, 1mg / ml, 0.5mg / ml, 0.1mg / ml, 0.05mg / ml in turn, respectively in Quejia Detection is carried out under separation and detection conditions, and linear regression is carried out according to the peak area (Y) and the concentration (X) of the corresponding sugar and sugar alcohol, and the standard curve, standard curve equation, linear range of 13 kinds of sugar and sugar alcohol can be obtained, and the concentration Continuously dilute, when the signal-to-noise ratio is 3, determine the detection limit of each sugar or sugar alcohol standard solution, the specific results are shown in Table 1.
[0023] Table 1 Standard curve equation, linear range, linear correlation coefficient and detection limit of thirteen kinds of sugar and sugar alcohol mixed standard solutions
[0024]
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Abstract
The invention belongs to the field of detection of saccharides and sugar alcohols in the food. An HPLC (high performance liquid chromatography) evaporative light scattering detection process for separating and detecting thirteen saccharides and sugar alcohols simultaneously comprises the following steps: gradiently eluting and separating a mixed standard solution of thirteen saccharides and sugar alcohols on an amino column through a mixed solution of acetonitrile and water as a separated flow phase with the eluting speed of 0.8 to 1.2ml / min; detecting the mixed standard solution after separation through an evaporative light scattering detector; and the detection conditions of the evaporative light scattering detector are as follows: the temperature of a drift tube is 40 to 70 DEG C, the carrier gas is N2, the flow velocity is 0.8 to 1.5l / min, and the gain is 1. The invention provides an analytic process for simultaneously separating and detecting multiple saccharides and sugar alcohols with high efficiency, convenience, low detection limit and high popularity.
Description
technical field [0001] The invention belongs to the detection field of sugar and sugar alcohol in food, in particular to glucose, fructose, xylose, maltose, sucrose, lactose, kestose, kestose, kestose, erythritol, Thirteen common sugars and sugar alcohols, xylitol, mannitol, and maltitol, were detected by high performance liquid chromatography evaporative light scattering. Background technique [0002] Sugar is one of the main nutrients in food, which mainly affects the texture, flavor, sweetness and other properties of food. At the same time, sugar is also the main source of energy for the human body. Common sugars in food include monosaccharides, such as xylose, glucose, fructose, disaccharides, such as maltose, lactose, sucrose, functional oligosaccharides, such as fructooligosaccharides (including kestose, kestose, tetraose, Cane Pentaose), functional sugar alcohols, such as erythritol, xylitol, mannitol, maltitol, etc. Among them, monosaccharides and disaccharides are...
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