Biocompatible nano composite drug carrier with synergistic anti-tumor effect, drug with synergistic anti-tumor effect and preparation methods of biocompatible nano composite drug carrier and drug

A nano-drug carrier, anti-tumor technology, applied in anti-tumor drugs, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problems of tissue lethality and low distribution.

Active Publication Date: 2013-02-13
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing anticancer drugs have high killing intensity on cancer cells, but they

Method used

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  • Biocompatible nano composite drug carrier with synergistic anti-tumor effect, drug with synergistic anti-tumor effect and preparation methods of biocompatible nano composite drug carrier and drug
  • Biocompatible nano composite drug carrier with synergistic anti-tumor effect, drug with synergistic anti-tumor effect and preparation methods of biocompatible nano composite drug carrier and drug
  • Biocompatible nano composite drug carrier with synergistic anti-tumor effect, drug with synergistic anti-tumor effect and preparation methods of biocompatible nano composite drug carrier and drug

Examples

Experimental program
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Effect test

preparation example Construction

[0059] Preparation steps of composite drug loading

[0060] Such as figure 1 As shown, first, select calcium carbonate nanoparticles in a certain size range, put them into an aqueous solution of a certain concentration of doxorubicin for adsorption, centrifuge, wash, and remove unadsorbed doxorubicin; then add polysaccharide solution, and adsorb for a period of time Finally, centrifuge, clean, and remove the unadsorbed polysaccharide solution; then put it into the aqueous solution of TRAIL, after a period of adsorption, centrifuge, clean, and remove the unadsorbed TRAIL solution; repeat the above steps several times, and the phospholipid The nanocomposite drug-loading system was prepared by wrapping TRAIL and TRAIL on the surface of calcium carbonate nanoparticles loaded with doxorubicin by alternate adsorption.

Embodiment 1

[0061] Embodiment 1, preparation has the medicine of synergistic antitumor effect

[0062] Get 0.5g of calcium carbonate particles with a size of 500-800nm ​​(see figure 2), add 1mg / mL doxorubicin aqueous solution, absorb for 8h, centrifuge for 3min, remove the unadsorbed doxorubicin solution in the upper layer, wash 2-3 times; then add 1mg / mL sodium alginate (ALG) solution , after 20 minutes of adsorption, centrifuge for 3 minutes, remove the excess sodium alginate sodium chloride solution in the upper layer, wash 23 times; TRAIL buffer solution, wash 2-3 times, then add 1 mg / mL sodium alginate (ALG) solution, absorb for 20 minutes, centrifuge for 3 minutes, remove excess sodium alginate in the upper layer of sodium chloride solution, wash 2-3 times; repeat the above-mentioned process of adsorbing TRAIL-sodium alginate 4 times, and finally obtain calcium carbonate particles adsorbing doxorubicin wrapped in 5 TRAIL / ALG (see image 3 ).

[0063] Get a certain amount (20mg) ...

Embodiment 2

[0068] According to the method of the present invention, the sodium alginate solution in Example 1 was replaced with heparin sodium solution of the same concentration, and the nanocomposite drug carrier was constructed and cytotoxicity experiments were carried out based on the same operation and principle. The result was similar to Example 1, without significant difference between the two.

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Abstract

The invention discloses a biocompatible nano composite drug carrier with a synergistic anti-tumor effect, a drug with a synergistic anti-tumor effect, and preparation methods of the biocompatible nano composite drug carrier and the drug. The biocompatible nano composite drug carrier provided by the invention comprises a core, a connecting layer coating the core and a shell layer coating the connecting layer, wherein the core is provided with degradable porous inorganic nano particles already absorbing anti-cancer drugs; the connecting layer is formed by polyelectrolyte; the shell layer is alternately assembled by protein and polyelectrolyte layer by layer; and the protein is selected from one tumor necrosis factor superfamily member. The invention has the advantages that by utilizing a layer-by-layer assembly technology, a great quantity of protein/polysaccharide nano composite drug carriers with core-shell structures can be prepared simply and conveniently; and the nano composite drug carrier can be used for wrapping and releasing water-soluble drugs, so that the cost is effectively reduced and scale production is easy to realize.

Description

technical field [0001] The invention relates to a biocompatible nano medicine composite carrier with synergistic anti-tumor effect, medicine and a preparation method thereof. Background technique [0002] A drug carrier is a system that can change the way a drug enters the body and its distribution in the body, controls the release rate of the drug, and delivers the drug to the target organ. As the material of drug sustained-release carrier, it is an important part of the drug sustained-release system, an important substance to regulate the drug release rate, and the main factor affecting the efficacy of the drug. It needs to be biocompatible and biodegradable, that is, it can be degraded into small molecular compounds in the body, so that it can be metabolized, absorbed or excreted by the body, has no toxic side effects on the human body, and the timing of the degradation process should be appropriate. After the drug is transported by the carrier, the damage should be smal...

Claims

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Application Information

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IPC IPC(8): A61K47/36A61K38/19A61P35/00
Inventor 李峻柏崔巍
Owner INST OF CHEM CHINESE ACAD OF SCI
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