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Alkaloid compound and preparation method and application of alkaloid compound

A technology of alkaloids and compounds, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as drugs that have not yet been seen, and achieve the effect of inhibiting cell activity

Active Publication Date: 2015-02-25
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on the chemical structure and cell proliferation inhibitory activity of this compound, so there are no related drugs on the market.

Method used

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  • Alkaloid compound and preparation method and application of alkaloid compound
  • Alkaloid compound and preparation method and application of alkaloid compound
  • Alkaloid compound and preparation method and application of alkaloid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The structural formula of an alkaloid compound is shown in I.

[0023]

Embodiment 2

[0025] The preparation method of the alkaloid compound shown in formula I specifically includes the following steps:

[0026] (1) Fermentation production

[0027] Pseudoalteromonas rubra.QD1-2 (Pseudoalteromonas rubra.QD1-2) was inoculated on Zobell 2216E solid slant medium by streaking it with the preservation number of CGMCC No.6555, and then cultured in an incubator at 30°C for 1 day; inoculating one colony obtained from slant culture The colonies were placed in 400ml EM medium and cultured on a shaker at 30°C and a rotating speed of 150rpm for 7 days to obtain a fermentation product;

[0028] (2) Obtaining extract

[0029] Centrifuge the fermentation product with a centrifuge to remove the bacteria to obtain the fermentation broth. Take the fermentation broth and add an equal volume of ethyl acetate to fully extract once, collect the extract, and then add the raffinate to an equal volume of ethyl acetate and repeat the extraction once. , Collect the extract, add the obtained raff...

Embodiment 3

[0041] In vitro anti-tumor activity test (cell proliferation inhibitory activity test)

[0042] (1) Experimental samples

[0043] Preparation of the test sample solution The test sample is the pure compound I separated and purified in the above-mentioned Example 1. An appropriate amount of the sample is accurately weighed, and a solution of the required concentration is prepared with methanol to test the activity.

[0044] The cell line and cell subculture adopt human gastric adenocarcinoma BGC-823 cells, human cervical cancer Hela cells and human liver cancer HepG2 cells and other cancer cell lines. All cells use 10% BCS 1640 medium at 37℃. Enter 0.5% carbon dioxide incubator for relay culture.

[0045] (2) Experimental method

[0046] Cell proliferation inhibitory activity test method: the tetrazolium salt (MTT) method takes human gastric adenocarcinoma BGC-823 cells, human cervical cancer Hela cells and human liver cancer HepG2 cells in logarithmic growth phase, and adjusts the dens...

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Abstract

The invention discloses an alkaloid compound, and a preparation method and an application of the alkaloid compound. The structure of the alkaloid compound is expressed as (I). The preparation method comprises the following steps that scratching and inoculating pseudoalteromonas nigrifaciens with the preservation number of CGMCC No. 6555 to a solid bevel culture medium, keeping the temperature at 30 DEG C, culturing the pseudoalteromonas nigrifaciens for one day, inoculating one colony into an EM (effective microorganisms) culture medium, keeping the temperature at 30 DEG C, culturing in a rocking bed with rotation speed of 150 rpm for seven days, and then obtaining a fermented product; removing thalli from the fermented product, obtaining the fermenting liquid, adding ethyl acetate with same volume as the fermenting liquid into the fermenting liquid to fully extract for three times, combing the extracting liquids, removing the ethyl acetate by a rotary evaporator, and obtaining a crude extract; and finally, dissolving the crude extract by methanol, separating and purifying by a semi-preparation reverse-phase high-efficiency liquid chromatogram, and then obtaining the alkaloid compound. The alkaloid compound has the advantage that the alkaloid compound has the functions of inhibiting cell activity and resisting tumor.

Description

Technical field [0001] The invention relates to an alkaloid compound, in particular to an alkaloid compound and a preparation method and application thereof. Background technique [0002] Indole alkaloids are the largest family of alkaloids, which can treat a wide range of diseases such as respiratory, digestive, cardiovascular, central nervous and tumors. At present, it is mainly extracted from plants and rarely found in bacteria. The inventors learned that the marine bacteria Pseudoalteromonas rubra.QD1-2 (deposited at the Third Institute of Oceanography, State Oceanic Administration, deposit number: CGMCC No.1k00044) liquid fermentation product was extracted with ethyl acetate, and the extract was very good The cell proliferation inhibitory activity of, so its active ingredients were studied. There is no report on the chemical structure and cell proliferation inhibitory activity of the compound, so there is no related drug on the market. Summary of the invention [0003] The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/14A61K31/4045A61P35/00C12P17/10C12R1/01
Inventor 何山严小军丁立建
Owner NINGBO UNIV
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