A kind of biological pesticide and insect control method
A biopesticide and lepidopteran insect technology, applied in botany equipment and methods, insecticides, biocides, etc., can solve the problem of slow effect of engineering strains expressing dsRNA, food safety problems of transgenic plants, high cost of dsRNA synthesis, etc. problem, to achieve the effect of short cycle, enhanced stability and effect, and simple method
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Embodiment 1
[0034] This example specifically provides the siRNA of the mitochondrial complex III Fe-S subunit gene of Plutella xylostella, which has a fatal effect on Lepidoptera insects such as Plutella xylostella after naturally feeding the siRNA of the target gene.
[0035] This example is a method for controlling the lepidopteran insect diamondback moth (Plutella xylostella). By feeding siRNA containing the silencing mitochondrial complex III Fe-S subunit gene (GeneBank accession number EU815629), the insect mitochondrial electron transfer is blocked, and the formation of insect ATP is inhibited, thereby achieving effective control of insects.
[0036] Mitochondrial complex III, also known as ubiquinol-cytochrome c reductase, consists of 9-11 subunits, including two cytochrome b (b562 and b566), one cytochrome C1 and one Fe- S protein. Among them, the Fe-S protein subunit is responsible for transferring an electron from reduced coenzyme Q to cytochrome C1, and releasing a proton to t...
Embodiment 2
[0068] This example specifically provides siRNA of the mitochondrial complex III Fe-S subunit gene of Plutella xylostella, by naturally feeding the target gene Si-UQCR_001 (SEQ ID NO 5 and 6) siRNA with cationic polymer polyethyleneimine (PEI ), chitosan, polylysine and gelatin have caused fatal effects on lepidopteran insects such as diamondback moth.
[0069]Si-UQCR_001 (SEQ ID NO 1 and 2) siRNA and the above cationic polymers were prepared into biopesticides, and evenly coated on cabbage leaves and fed to the second instar larvae of Plutella xylostella. In groups 1-4, N in biopesticides :P is 1:1, and in groups 5-6, N:P in biopesticides is 20:1. The leaf concentration of siRNA was 3.0 μg / cm 2 . 30 worms were treated with each pair of siRNA, and three replicates were set up, and DEPC water was used as a negative control group. After that, the number of dead insects was investigated respectively at 12h, 24h, 36h, 48h, 60h, and 72h, and the mortality rate was calculated. Th...
Embodiment 3
[0075] This example specifically provides siRNA of the acetylcholinesterase gene of diamondback moth, which has caused fatal effects on lepidopteran insects such as diamondback moth after natural feeding of the siRNA of the target gene.
[0076] This example is a method for controlling the lepidopteran insect diamondback moth (Plutella xylostella). By feeding siRNA containing silencing acetylcholinesterase genes 1 and 2 (GeneBank accession numbers: AY970293 and AY061975), the normal nerve impulse transmission of insects is affected, and the insects continue to be in a state of excitement and die, thereby realizing the effective treatment of insects. governance.
[0077] Acetylcholinesterase is a serine hydrolase whose main function is to stop the transmission of nerve impulses by rapidly hydrolyzing the neurotransmitter acetylcholine (ACh) at cholinergic synapses (Taylor T, Radie Z, 1994. Thecholinesterases: from genes to proteins.Annu Rev Pharmaco1.Toxico1., 34:281-320). Th...
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