Unlock instant, AI-driven research and patent intelligence for your innovation.

Inoculation operation method for reducing contamination rate of cultivating bottle

An operation method and cultivation bottle technology, applied in the field of strain inoculation operation, can solve the problems of reducing the output of edible fungi, secondary pollution of cultivation bottles, affecting the quality of mycelium, etc., and achieve low implementation cost, improved survival rate, and simple structure Effect

Inactive Publication Date: 2013-03-20
SHANGHAI XUERONG BIOTECH
View PDF13 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after using the liquid strain delivery pipeline for a period of time, the nozzle of the liquid strain delivery pipeline is easily contaminated with bacteria, and the solution of the liquid strain contains rich nutrients. Once the bacteria are adsorbed on the nozzle, it can be used The solution of the liquid strain contaminated on the nozzle is rapidly propagated, and the liquid strain output from the liquid strain delivery pipeline is directly polluted, and after the cultivation bottle is inoculated with this liquid strain, in addition to causing damage to the cultivation bottle In addition to avoiding secondary pollution, it will also affect the quality of mycelia after inoculation and reduce the yield of edible fungi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Inoculation operation method for reducing contamination rate of cultivating bottle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Such as figure 1 As shown, when the liquid strain is inoculated, the seed tank containing the liquid strain is first inflated, so that the liquid strain gushes out of the seed tank, and the liquid strain is output from the liquid strain delivery pipeline connected to the seed tank to the liquid strain At the nozzle connected to the delivery pipeline, the nozzle sprays the liquid bacteria into the cultivation bottle, and one time of liquid bacteria inoculation can be completed.

[0029] Then close the strain solenoid valve through the microprocessor system, thereby disconnecting the seed tank and the liquid strain delivery pipeline, and first carry out alcohol disinfection on the nozzle by the alcohol spray device, and use the characteristics of alcohol to kill most of the bacteria attached to the nozzle. Then use the water pump to inject sterile water into the liquid strain delivery pipeline, control the flow rate of the sterile water within 0.5-1.5 meters per second, a...

Embodiment 2

[0032] During the liquid strain inoculation process, after the two liquid strains are inoculated, the liquid strain delivery pipeline is flushed with sterile water once.

[0033] When sowing liquid strains, the seed tank is first inflated, and the gas pressure is controlled at 0.1Mpa, so that the liquid strains in the seed tank flow into the liquid strain delivery pipeline, and the liquid strains are sprayed into the cultivation bottle through the nozzle.

[0034] After completing the above steps continuously for half an hour, close the strain solenoid valve through the microprocessor system, thereby disconnecting the seed tank and the liquid strain delivery pipeline, and then use the water pump to deliver sterile water to the liquid strain delivery pipe, and the sterile The flow rate of the bacterial water is controlled at 1.5-3 meters per second to achieve the effect of high-pressure flushing. The bacteria piles attached to the nozzles are strongly flushed, and the flushed st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of bioengineering, in particular to a strain inoculation operation method. The inoculation operation method for reducing the contamination rate of a cultivating bottle comprises the following steps: 1) strain spraying, 2 cleaning and degerming, 3) waste water treatment. By the adoption of the technique, the inoculation operation method for reducing the contamination rate of the cultivating bottle is simple in structure, low in constructing cost, and capable of effectively cleaning and degerming a liquid strain inoculation device, reducing the contamination rate of liquid strains, and improving rate of survival of strains.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a strain inoculation operation method. Background technique [0002] Edible fungi are one of the foods with high nutritional value that people like. In recent years, the artificial cultivation technology of edible fungi has developed greatly, and the cultivation technology of edible fungi has gradually changed from manual cultivation in the past to automation and industrialization. In the cultivation process of edible fungi, the inoculation process of edible fungus strains directly plays a vital role in the growth of edible fungi. [0003] At present, the strains of edible fungi are mainly divided into solid strains and liquid strains. Compared with solid strains, liquid strains have the advantages of shorter cultivation time, faster germination, high biotransformation rate, and higher purity of strains. It is convenient for large-scale and continuous production, which is the trend...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01G1/04
Inventor 问卓君
Owner SHANGHAI XUERONG BIOTECH