Method for preparing nano aluminum hydroxide gel adjuvant

Abstract

The invention relates to a method for preparing a nano aluminum hydroxide gel adjuvant, belonging to the technical field of processes for preparing immunologic adjuvant of biological products for animals. The method comprises the following steps: mixing an aluminum salt solution and a soda solution which serve as raw materials, and adding a certain amount of stabilizing agent during mixing; shearing by a high-shearing emulsifying machine to obtain the nano aluminum hydroxide solution preliminarily; precipitating, washing and re-precipitating to obtain nano aluminum hydroxide gel; preparing a solution from the nano aluminum hydroxide; and compounding the solution with an inactivated bacterial solution to obtain an inactivated vaccine. The nano aluminum hydroxide gel adjuvant prepared by adopting the method is uniform in particle size distribution, has high purity, strong adsorbability and no side effect and has a good application prospect in the aspect of preparation of immunologic adjuvant.

Description

technical field [0001] The invention belongs to the technical field of preparation technology of animal biological products immune adjuvant, and in particular relates to a preparation method of aluminum hydroxide nanogel adjuvant. Background technique [0002] Adjuvants were first described by Gaston Ramon as a substance used in combination with specific antigens which produced a much higher immune response than when used alone. In general, adjuvants do not provide immunity by themselves; only when combined with an immunogen in a formulation can they improve the breadth and potency of the immune response induced by a vaccine. [0003] Most of the immunological adjuvants used in clinical practice are white oil adjuvant, aluminum hydroxide adjuvant, lipid adjuvant and propolis adjuvant. White oil adjuvant has good immunogenicity, but has high toxicity and side effects; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but cannot induce cellular i...

AI Technical Summary

Problems solved by technology

White oil adjuvant has good immunogenicity, but has high toxicity and side effects; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but cannot induce cellular immunity; lipid adjuvant and propolis adjuvant have good immunogenicity, and can induce humoral Immunity and cellular immunity, the duration of immunity is long, but the raw materials are expensive

Therefore, in recent years, researchers from various countries have developed a series of new immune adjuvants using different raw materials and methods, but none of them have been widely used because of unsatisfactory effects or high toxicity.