Method for preparing nano aluminum hydroxide gel adjuvant

A technology of aluminum hydroxide and nano glue, which is applied in the direction of aluminum oxide/aluminum hydroxide, medical preparations containing active ingredients, pharmaceutical formulas, etc., which can solve the problems of inability to induce cellular immunity, long duration of immunity, and expensive raw materials , to achieve the effects of prolonging the duration of rabbit plague, improving quality and uniform particle size distribution

Active Publication Date: 2013-03-27
山东滨州沃华生物工程有限公司
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  • Abstract
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Problems solved by technology

White oil adjuvant has good immunogenicity, but has high toxicity and side effects; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but cannot induce cellular immunity; lipid adjuvant and propolis adjuvant have good immunogenicity, and can induce humoral Immunity and cellular imm...

Method used

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Embodiment Construction

[0017] In order to make the technical solution of the present invention clearer, the present invention will be further described below in conjunction with examples of inactivated vaccines for infectious rhinitis.

[0018] 1. Vaccine preparation

[0019] 1. Preparation of antigen:

[0020] (1) Bacteria culture: Inoculate the production seeds in the semi-synthetic medium, seal the bottle mouth and shake well, put it in a greenhouse at 37°C for static culture for about 18 hours, and shake it every 6-8 hours during the period. When the bacterial liquid is turbid and light yellow, it reaches the peak of growth and is ready to be harvested. At this time, the bacterial content can reach about 1 billion / ml.

[0021] (2) Concentration and inactivation of the bacteria solution for making vaccines: use a concentrator to concentrate the purely qualified bacteria solution, and then make a suspension with physiological saline with a pH of 7.2 to make 4 to 5 billion / ml bacteria, The final...

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Abstract

The invention relates to a method for preparing a nano aluminum hydroxide gel adjuvant, belonging to the technical field of processes for preparing immunologic adjuvant of biological products for animals. The method comprises the following steps: mixing an aluminum salt solution and a soda solution which serve as raw materials, and adding a certain amount of stabilizing agent during mixing; shearing by a high-shearing emulsifying machine to obtain the nano aluminum hydroxide solution preliminarily; precipitating, washing and re-precipitating to obtain nano aluminum hydroxide gel; preparing a solution from the nano aluminum hydroxide; and compounding the solution with an inactivated bacterial solution to obtain an inactivated vaccine. The nano aluminum hydroxide gel adjuvant prepared by adopting the method is uniform in particle size distribution, has high purity, strong adsorbability and no side effect and has a good application prospect in the aspect of preparation of immunologic adjuvant.

Description

technical field [0001] The invention belongs to the technical field of preparation technology of animal biological products immune adjuvant, and in particular relates to a preparation method of aluminum hydroxide nanogel adjuvant. Background technique [0002] Adjuvants were first described by Gaston Ramon as a substance used in combination with specific antigens which produced a much higher immune response than when used alone. In general, adjuvants do not provide immunity by themselves; only when combined with an immunogen in a formulation can they improve the breadth and potency of the immune response induced by a vaccine. [0003] Most of the immunological adjuvants used in clinical practice are white oil adjuvant, aluminum hydroxide adjuvant, lipid adjuvant and propolis adjuvant. White oil adjuvant has good immunogenicity, but has high toxicity and side effects; aluminum hydroxide gel adjuvant has good immunogenicity and small side effects, but cannot induce cellular i...

Claims

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Application Information

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IPC IPC(8): A61K39/39C01F7/02
Inventor 郎景民马景霞张静
Owner 山东滨州沃华生物工程有限公司
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