Klebsiella variicola with potassium release function, and culture method and application thereof

A technology of Klebsiella and culture method, applied in the field of biological strains, to achieve the effect of improving the quality of tobacco leaves, reducing the amount of application, and increasing the potassium content

Inactive Publication Date: 2013-04-10
TOBACCO RES INST CHIN AGRI SCI ACAD +2
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on potassium-decomposing bacteria in tobacco rhizosphere, and there are few reports

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Klebsiella variicola with potassium release function, and culture method and application thereof
  • Klebsiella variicola with potassium release function, and culture method and application thereof
  • Klebsiella variicola with potassium release function, and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1 Isolation of potassium dissolving bacteria in tobacco rhizosphere

[0027] 1. Potassium dissolving bacteria culture medium: NaH 2 PO 4 2.0g, MgSO 4 ·7H 2 O 0.2g, FeCl 3 0.05g, sucrose 5.0g, CaCO 3 0.1g, 1.0g potassium feldspar powder, 15-20g agar, 1000mL distilled water, pH 7.0-7.5, sterilized at 121°C for 15 minutes for use.

[0028] 2. Beef extract peptone culture medium: beef extract 3g, peptone 5g, agar 18-20g, distilled water 1000mL, pH 7.0-7.2, sterilized at 121°C for 15 minutes for use.

[0029] The potassium-dissolving bacteria medium was used to screen potassium-dissolving bacteria in the soil. Take 5g of rhizosphere soil sample and dissolve it into 100mL sterile water to make 10 -3 ~10 -6 Dilution degree rhizosphere bacteria suspension, take 10 -4 ~10 -6 Diluted Rhizosphere bacteria suspension 50μl in Aleksandrov medium (sucrose 5g, MgSO 4 ·7H 2 O 0.5g, FeCl 3 0.005g, NaH 2 PO 4 ·12H 2 O 2g, CaCO 3 0.1g, 20g agar powder, 1000mL of distilled water, pH 7...

Example Embodiment

[0033] Example 2 Identification of Trb33 strain

[0034] Refer to the method of Kim et al. and Rainey et al. to extract a small amount of total DNA from the Trb33 strain in Example 1. Use a sterile toothpick to pick a small amount of purified bacteria and resuspend in 2μL sterile ddH 2 After boiling at 100°C for 10 minutes in O, quickly ice bath for 2 minutes, and centrifuge at 12000r / min at 4°C for 5 minutes. To ensure the PCR effect, centrifuge once before each use. Take 3μL of supernatant for 50μL PCR reaction system. The upstream primer used in the reaction is: 5'-AGAGTTTGATCATGGCTCAG-3', and the downstream primer used in the reaction is: 5'-TACGGTTACCTTGTTACGACTT-3'. PCR amplification system (50μL): 0.5μL genomic DNA, 2.5mmol / L dNTP mixture 4μL, forward and reverse primers 1μL each, 0.5μL TansTaqDNA polymerase (Fermentas), 5μL reaction buffer (10×buffer), ddH 2 O38μL. PCR reaction program: 1 min at 94℃, 30s at 94℃, 30s at 48℃, 5min at 72℃, 10min at 72℃, 30 cycles.

[0035]...

Example Embodiment

[0040] Example 3 Determination of Potassium Resolving Activity of Trb33 Strain

[0041] Further analysis of the ability of the strains showing dissolving circles on the potassium-dissolving medium in Example 2 to release potassium from the liquid medium. Add the prepared Aleksandrov liquid medium (without agar) into a 250mL Erlenmeyer flask according to the volume of each bottle of 50mL, 0.1MPa, 20min sterilization, after cooling, add 1mL of potassium-dissolving bacteria solution, and set a blank for no inoculation Contrast. After inoculation, culture was shaken at 28±1°C and 150rpm for 7 days. The experiment was set to repeat 3 times. On the 7th day, the content of available potassium in the culture solution was measured.

[0042] Determination of available potassium in supernatant

[0043] Pour out the potassium bacteria fermentation broth cultured in the Erlenmeyer flask and dilute to 50 mL, centrifuge the fermentation broth at 500 rpm for 10 minutes to remove the insoluble subs...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a Klebsiella variicola Trb33 strain with relatively high potassium release function, a culture method of the Klebsiella variicola Trb33 strain, and an application of the strain in promoting tobacco growth. The Klebsiella variicola Trb33 disclosed by the invention can be used for the preparation of silicate microbial fertilizer, which decomposes silicate minerals of potassium feldspar, mica and the like in soil to release soluble potassium for the tobacco to adsorb and use, thus the application amount of chemical potassium fertilizer is reduced, the soil micro-environment is improved, and the tobacco quality is improved.

Description

technical field [0001] The invention relates to the technical field of biological strains, in particular to Klebsiella mutabilis having the function of dissolving potassium, and a cultivation method and application of the Klebsiella mutans. Background technique [0002] Tobacco is a potassium-loving crop. Potassium content is one of the important indicators for judging the quality of tobacco leaves. Potassium in tobacco not only plays a role as an important nutrient element, but also improves the combustibility of tobacco leaves, thereby reducing the amount of tar produced, and improving the safety of tobacco products. sex. Potassium can participate in almost all metabolic processes in tobacco plants (He Nianzu et al., 1987), and can greatly affect the energy and substance metabolism of tobacco plants. At present, the potassium content of tobacco leaves in our country is low, and the production is mainly based on the application of chemical potassium fertilizers. By increas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C05G3/00C12R1/22
Inventor 张忠锋申国明祖秉桥李青诚高林张玉芹高加明张淼代光照
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap