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Method for improving cold and disease resistance of tobacco

A technology of disease resistance and tobacco, applied in the field of agricultural microbial applications, can solve the problems that mycorrhizal fungi cannot be directly cultured in vitro, the effect of mycorrhizal fungi is not very prominent, and the independent contribution of mycorrhizal fungi is low, so as to improve the rhizosphere Effects of micro-ecological environment, improvement of infestation status, and reduction of low temperature damage

Inactive Publication Date: 2012-08-01
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since mycorrhizal fungi cannot be directly cultured in vitro, the application of mycorrhizal fungi to tobacco is severely restricted. At present, inoculation of mycorrhizal fungi and tobacco seedlings is the preferred method for tobacco mycorrhization, but the field effect of mycorrhizal tobacco is often The effect of mycorrhizal fungi is not very prominent in the field, indicating that the independent contribution of mycorrhizal fungi to the rhizosphere microbial environment is low, and they must work together with other microorganisms
[0005] Although the normal growth of tobacco can be improved by selecting suitable tobacco varieties and adjusting the moisture, temperature or other cultivation conditions during the tobacco growth process, the selection of varieties often takes a long time and cannot meet the actual needs of current tobacco production.
Moreover, in the existing tobacco research, mycorrhizal fungi can improve tobacco drought resistance and reduce heavy metal absorption research reports; for a single mycorrhizal fungus or phosphorus-dissolving bacteria can improve tobacco growth or nutrient absorption under pot conditions, but the field effect is often not not ideal
There is no report for the interaction of two kinds of microbial agents in improving tobacco growth or disease resistance and cold resistance; and the microbial inoculation technology of mycorrhizal fungi and phosphorus-dissolving bacteria in improving tobacco growth, cold resistance and disease resistance has not yet been reported. No report

Method used

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  • Method for improving cold and disease resistance of tobacco

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]1. The preparation method of the solid bacterial agent of Glomus mosesi: take the sieved river sand as the substrate, after high-temperature sterilization (121 ℃ high-pressure steam sterilization for 45 minutes each time), leave it in the natural state for 8 days, and then pack it into two kilograms Put it into a pot, inoculate 100g of the original species of Glomus moses, mix well, add tap water with 25% of the substrate weight, sow the seeds of sorghum and scallions, keep normal growth, and get infected by Glomus moses after six months The host plant and the matrix containing Glomus moses spores and extra-root hyphae; the sorghum and scallions above the ground are cut off, the root segments are chopped, and mixed with the matrix containing Glomus moses spores and extra-root hyphae, That is, the solid inoculum of Glomus moxie was prepared. Each gram of Glomus mosesi solid inoculum contains 40 Glomus moses spores.

[0022] The Glomus mosesi solid inoculant was uniformly...

Embodiment 2

[0032] 1. The preparation method of Glomus moses solid inoculum: use sieved river sand as the substrate, after high-temperature sterilization (121 ℃ high-pressure steam sterilization for 45 minutes each time), leave it in the natural state for 7 days, and then pack it into two kilograms Put it in a pot, inoculate 100g of the original species of Glomus moses, mix well, add tap water with 20% of the weight of the substrate, sow the seeds of sorghum and green onions, keep normal growth, and get infected by Glomus moses after six months The host plant and the matrix containing Glomus moses spores and extra-root hyphae; the sorghum and scallions above the ground are cut off, the root segments are chopped, and mixed with the matrix containing Glomus moses spores and extra-root hyphae, That is, the solid inoculum of Glomus moxie was prepared. Each gram of Glomus mosesi solid inoculum contains 40 Glomus moses spores.

[0033] The Glomus mosesi solid inoculant is uniformly mixed with ...

Embodiment 3

[0041] 1. The preparation method of the solid bacterial agent of Glomus mosesi: take the sieved river sand as the substrate, after high-temperature sterilization (121 ℃ high-pressure steam sterilization for 45 minutes each time), place it in the natural state for 10 days, and then take two kilograms Put it into a pot, inoculate 100g of the original species of Glomus moses, mix well, add 30% of the substrate weight in tap water, sow the seeds of sorghum and scallions, keep normal growth, and get infected by Glomus moses after six months The host plant and the matrix containing Glomus moses spores and extra-root hyphae; the sorghum and scallions above the ground are cut off, the root segments are chopped, and mixed with the matrix containing Glomus moses spores and extra-root hyphae, That is, the solid inoculum of Glomus moxie was prepared. Each gram of Glomus mosesi solid inoculum contains 40 Glomus moses spores.

[0042] The Glomus mosesi solid inoculant is uniformly mixed wi...

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PUM

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Abstract

The invention discloses a method for improving cold and disease resistance of tobacco, which includes: raising tobacco seedlings by floating process, inoculating mycorrhizal fungi 2-3cm below sowing positions of tobacco seeds in each foamed floating porous seedling tray filled with substrate, guaranteeing each single tobacco plant to contain 90-150 spores of the mycorrhizal fungi, sowing the tobacco seeds, cultivating, and waiting for tobacco seedlings to grow up; and inoculating phosphate-solubilizing microorganisms liquid inoculant for two to five times in two weeks before transplanting of the tobacco seedlings, guaranteeing inoculation amount to be 103-105 phosphate-solubilizing microorganisms per tobacco seedling each time, transplanting the tobacco seedlings, covering with earth, andmanaging the tobacco seedlings conventionally. By using the method to treat tobacco, the cold and disease resistance of the tobacco is improved, and nutrition and growth of the tobacco can also be improved.

Description

Technical field: [0001] The invention belongs to the application field of agricultural microorganisms, and in particular relates to a method for improving tobacco cold resistance and disease resistance. Background technique: [0002] Flue-cured tobacco is one of the main economic crops in my country and the most important basic raw material for the cigarette industry. Flue-cured tobacco cultivation implements a regionally centralized and highly unified production management system. With the high degree of planting intensification, the soil for tobacco production is further deteriorated, especially the continuous cropping obstacles are becoming more and more serious. As cigarettes are special consumer goods, the quality requirements are very high, and its quality is directly related to the income of tobacco farmers. Soil quality will not only affect the yield of tobacco, but also affect the quality of tobacco, so that tobacco is restricted by "grade", and further restricts t...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06
Inventor 丁效东李淑仪詹振寿王军
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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