Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for enhancing soil fertility by promoting straw degradation through protease

A technology of soil fertility and protease, which is applied in the field of biological enzyme application and soil fertilizer, can solve the problems of affecting the growth of seedlings, affecting the yield of later seasons, and short intervals, and achieves the effects of low cost, alleviating nitrogen starvation, and simple methods

Inactive Publication Date: 2015-01-07
NANJING UNIV OF INFORMATION SCI & TECH
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the interval between rice-wheat rotation or wheat-corn rotation is short, and nitrogen fixation seriously affects the growth of seedlings, which in turn affects the yield of later seasons.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] AS1.398 neutral protease (Bacillus subtilis neutral protease), whose protease activity is 58,820 U / g, the optimum reaction pH value is 6.5-7.5, and the optimum reaction temperature is 35-45°C, was purchased from Beijing Fangshan Enzyme Preparation main plant.

[0025] The soil samples came from the paddy field soil in Wujing Town, Minhang District, Shanghai, and the rice straw and soil were taken from the same plot. Under the condition of daily average temperature of 25-29℃, the soil samples were treated with enzyme treatment method and control treatment method respectively.

[0026] Enzyme treatment:

[0027] (1) The rice straw is crushed into small pieces with a length of 1 mm to obtain the crushed straw; the usage amount of the straw is 333 kg / mu.

[0028] (2) The AS1.398 neutral protease was prepared into a protease solution with a concentration of 5882U / g, and the straw was sprayed and crushed. Mix the crushed straw and enzyme liquid evenly. The protease activi...

example 2

[0034] AS1.398 neutral protease (Bacillus subtilis neutral protease) with a protease activity of 58,820 U / g was selected and purchased from Beijing Fangshan Enzyme General Factory.

[0035] Soil samples came from paddy field soil in Wujing Town, Minhang District, Shanghai, and wheat straw and soil were taken from the same plot. Under the condition of daily average temperature of 25-29 ℃, the soil samples were treated with enzyme treatment method and control treatment method.

[0036] Enzyme treatment:

[0037] (1) The wheat straw was crushed into small pieces with a length of 1 mm to obtain the crushed straw; the usage amount of the straw was 333 kg / mu.

[0038] (2) The AS1.398 neutral protease was prepared into a protease solution with a concentration of 5882U / g, and the straw was sprayed and crushed. Mix the crushed straw and enzyme liquid evenly. The protease activity in the enzyme solution sprayed per gram of crushed straw was 588U.

[0039] (3) Bury the crushed straw ...

example 3

[0044] The protease used was AS1.398 neutral protease (Bacillus subtilis neutral protease), with an activity of 50,000 U / g, which was purchased from Beijing Fangshan Enzyme General Factory.

[0045] The soil samples came from the experimental field of Shandong Agricultural University in Tai'an City, Shandong Province, and the corn stalks and soil were taken from the same plot. Under the condition of daily average temperature of 20-25 ℃ (in the greenhouse), the soil samples were treated with enzyme treatment method and control treatment method respectively.

[0046] The enzyme treatment method is as follows:

[0047] (1) Corn stalks are cut into small pieces with a length of 2 cm to obtain crushed stalks; the proportion of stalks used is 500 kg / mu.

[0048](2) Prepare AS1.398 neutral protease into a protease solution with a concentration of 5000U / g, and spray and crush the straw. Mix the crushed straw with the enzyme liquid evenly. The protease activity in the enzyme solutio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for enhancing soil fertility by promoting straw degradation through protease, and relates to the technical field of bio-enzyme application and soil fertilizer. The method comprises the steps of at daily mean temperature of 20-29 DEG C, (1) slicing and grinding the straw into small sections of 0.1-8cm to obtain ground straw; (2) spraying a protease solution onto the ground straw; and (3) embedding the ground straw sprayed with the protease solution into the soil. Through returning the straw added with the protease solution into the field, the method for enhancing the soil fertility disclosed by the invention solves the problems of reduced nitrogen content, barrier to crop growth and the like appearing in the early stage of returning the straw into the field, and improves the nitrogen content in the soil, enhances soil fertility, improves crop growth environment, turns waste into valuable things, and reduces the cost.

Description

technical field [0001] The invention relates to the technical field of biological enzyme application and soil fertilizer, in particular to a method for enhancing soil fertility by using protease to promote straw degradation. Background technique [0002] my country is a large agricultural country with abundant straw resources. The annual output of straw in my country is about 700 million tons. Straw returning to the field is an important part of ecological agriculture and circular agriculture. However, in recent years, with the development of the rural economy, clean and convenient petrochemical energy has replaced traditional crop straws. About 2 / 3 of the total straw is burned directly in the country every year, which accelerates soil degradation and causes soil to compensate for the lack of organic matter. And soil failure, soil ecological environment into a vicious circle. The pollution of chemical fertilizers to the environment is becoming more and more serious, resulti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/00
Inventor 韩玮何明聂俊华
Owner NANJING UNIV OF INFORMATION SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com