Use of salvianolic acid a freeze-dried powder injection for the preparation of drugs for preventing and/or treating cerebral thrombosis
A technology of freeze-dried powder injection and salvianolic acid, which is applied in the field of preparation of drugs for the prevention and/or treatment of cerebral thrombosis, can solve the problems of instability of injections and the inability to guarantee the pharmacological effects of salvianolic acid A, and achieve the effect of improving the conversion rate
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Embodiment 1
[0095] Take Danshen medicinal material, crush it into 6-mesh granules, add 7 times the amount of 92°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to relative Density 1.20 (60°C), add ethanol to make the alcohol content at 70%, let it stand, filter, the filtrate is decompressed to recover ethanol and concentrate until it has no alcohol smell; add water to dilute to contain salvianolic acid B 20mg per 1ml, and use 10 mg of salvianolic acid B in the aqueous solution % NaOH to adjust the pH to 4.0, add 0.5% ZnCl 2As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation, the ratio of the...
Embodiment 2
[0098] Take Salvia miltiorrhiza, crush it into particles with a diameter of about 2mm, add 7 times the amount of 90°C water each time, warm soak and extract 3 times, and stir at a speed of 25 rpm, and warm soak and extract for 3 hours each time; the extract is concentrated under reduced pressure to Relative density 1.16 (60°C), add ethanol to make the alcohol content 70%, let it stand, filter, the filtrate recovers ethanol under reduced pressure and concentrates until there is no alcohol smell; dilute with water to contain 20mg of salvianolic acid B per 1ml, for aqueous solution 10% sodium hydroxide to adjust pH to 3.5, add 0.5% ZnCl 2 As a catalyst, heat and transform at 120°C for 4 hours, adjust the pH value of the transformation liquid to 2.5 with 20% phosphoric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 3 mg of salvianolic acid A per 1 ml, and perform HPD-100 macroporous resin column chromatography For separation, the ratio of the loadi...
Embodiment 3
[0101] Take Salvia miltiorrhiza, cut into decoction pieces, add 8 times the amount each time, soak in water at 85°C for 3 times, stir at a speed of 20 rpm, and extract with warm soaking for 2.5 hours each time; the extract is concentrated under reduced pressure to a relative density of 1.20 (60 ℃), add ethanol to make the alcohol content at 75%, let it stand, filter, the filtrate recovers ethanol under reduced pressure and concentrates until it has no alcohol smell; add water to dilute to contain salvianolic acid B 15mg per 1ml, and use 10% potassium hydroxide in the aqueous solution Adjust pH to 5.0, add 0.6% ZnCl 2 As a catalyst, heat conversion at 120°C for 3.5 hours, adjust the pH value of the conversion liquid to 2.5 with 15% hydrochloric acid, centrifuge, concentrate the supernatant under reduced pressure to contain 5 mg of salvianolic acid A per 1 ml, pass through HPD-100 macroporous resin column layer Analysis and separation, the ratio of the loading amount of salviano...
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