Application of trepang sulphated polysaccharide in preparation of drugs or products for resisting diabetes mellitus II

A diabetes drug, technology of sulfated polysaccharides, applied in the improvement or treatment of type II diabetes, sea cucumber sulfate polysaccharides in the field of prevention, can solve the problem of no sea cucumber sulfate polysaccharides, achieve significant treatment of type II diabetes, eliminate side effects, increase glucose The effect of tolerance

Inactive Publication Date: 2013-05-22
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Application of trepang sulphated polysaccharide in preparation of drugs or products for resisting diabetes mellitus II
  • Application of trepang sulphated polysaccharide in preparation of drugs or products for resisting diabetes mellitus II
  • Application of trepang sulphated polysaccharide in preparation of drugs or products for resisting diabetes mellitus II

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The preparation method of embodiment 1 sea cucumber sulfate polysaccharide:

[0018] 1. Preparation of sea cucumber chondroitin sulfate and sea cucumber fucoidan sulfate: desilt and pulverize dried sea cucumber, degrease in 95% ethanol for 24 hours, and dry at room temperature. Add 3L 0.1M sodium acetate buffer solution (pH6.0), 10g papain, 2.92g EDTA solution and 1.21g cysteine ​​solution to 100g dry matter, stir and react in a water bath at 60°C for 24h, then centrifuge the reaction mixture (4000g , 15min). Add 160mL of 10% CPC solution to the supernatant, leave it at room temperature for 24h, centrifuge (3000g, 15min), and discard the supernatant. Dissolve the precipitate in 1.5L 3mol / L NaCl: ethanol (100:15v / v) solution, then add 3L 95% ethanol solution, place at 4°C for 24h, centrifuge (3000g, 15min), use 3L 80% and 95% ethanol for precipitation Wash two to three times. Finally, the precipitate was dried at 60° C. for 2 hours, dissolved in distilled water, ultra...

Embodiment 2

[0022] Example 2: Preventive and therapeutic effects of sea cucumber chondroitin sulfate on type II diabetic mice

[0023] one. Materials and methods:

[0024] A mouse model of type II diabetes was established by high-sugar and high-fat feeding. C57BL / 6 male mice weighing 19–21 g were randomly divided into 6 groups: normal control group, model control group, positive control group, sea cucumber chondroitin sulfate (SC-CHS) low and high dose groups, high dose sea cucumber Chondroitin sulfate and rosiglitazone compound group, 10 rats in each group. The normal control group was fed with normal feed, the protein content in the formula was 20%, the fat content was 5%, and the starch content was 65%, and the mineral and vitamin complexes were all referred to the AIN-93 formula; the mice in other groups were fed with high-sugar For high-fat feed, the protein content in the formula is 20%, the fat content is 25%, the sucrose is 30%, and the mineral and vitamin complexes are all ref...

Embodiment 3

[0071] Example 3 Capsule preparation

[0072] Get 100g of sea cucumber sulfated polysaccharide prepared in Example 1, 20g of micropowdered silica gel, 380g of dextrin, and 500g of starch, mix well and pack into capsules, each capsule is 1g, which contains sea cucumber sulfated polysaccharide 100mg.

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Abstract

The invention discloses an application of trepang sulphated polysaccharide in preparation of drugs, health food or and functional food for preventing, improving or treating diabetes mellitus II. The trepang sulphated polysaccharide is one or two of trepang chondroitin sulfate and trepang fucus chitosan sulfate. The animal experiment shows that the trepang sulphated polysaccharide obviously reduces the fasting plasma glucose of diabetic mice, improves the tolerance dose of oral glucose, reduces the insulin resistance index, and obviously raises and activates the PI3K/PKB/Glut4 signal transduction pathway mediated by the insulin. The trepang sulphated polysaccharide further obviously coordinates the antidiabetic drugs thiazolidinediones, and eliminates the side effects of glucose-lowering medications at the same time. The trepang sulphated polysaccharide disclosed by the invention has good effects on reducing the blood sugar and improving the resistance of the insulin, so that the trepang sulphated polysaccharide can be applied to development of the drugs and products for preventing, improving or treating the diabetes mellitus II.

Description

technical field [0001] The invention belongs to the field of health food and medicine, and in particular relates to the application of sea cucumber sulfate polysaccharide in preventing, improving or treating type II diabetes. Background technique [0002] Diabetes mellitus is a chronic endocrine and metabolic disease characterized by long-term hyperglycemia. It is the third leading cause of disabling and fatal diseases after disease and cancer. Diabetes is divided into type 1 diabetes (insulin-dependent diabetes) and type 2 diabetes (non-insulin-dependent diabetes). In recent years, with the development of the economy, the improvement of living standards and the change of residents' living patterns, the prevalence of diabetes has shown a global upward trend, of which type II diabetes accounts for more than 93.7% of the total diabetes. Insulin Resistance (Insulin Resistance) refers to the decreased sensitivity of insulin target organs to insulin action, that is, a state in ...

Claims

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Application Information

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IPC IPC(8): A61K31/737A61P3/10A23L1/30
Inventor 王静凤胡世伟常耀光龙腾腾石迪薛长湖王玉明
Owner OCEAN UNIV OF CHINA
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