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Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof

A technology of Chinese hairy sporophyte and proteoglycan, which is applied in the field of Chinese hairy sporophyte proteoglycan and its preparation, can solve problems that do not involve the identification of polysaccharide activity or advanced structure, and achieve low cost, simple operation, and low equipment requirements. Effect

Active Publication Date: 2013-06-05
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the above reports are about the extraction and separation of exopolysaccharides from Cordyceps sinensis, and do not involve the identification of polysaccharide activity or higher-order structures

Method used

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  • Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof
  • Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof
  • Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0044] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculum amount of 6% (weight percentage), and culture it statically for 40 days at 18°C ​​to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 20%, peptone 5%, cicada pupa powder 10%, KH 2 PO 4 1.0%, MgSO 4 .7H 2 O 0.5%, the balance is sterile water.

[0045] (2) Alcohol precipitation: Concentrate the Mortierella sinensis fermentation liquid in step (1) to obtain a concentrated liquid, add 4 times the volume of the concentrated liquid, and the volume percentage concentration is 95% ethanol aqueous solution, stir and mix, place in 4 Precipitate overnight in the refrigerator at ℃, then centrifuge at 6000rpm at 4℃ for 25min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis once;

[0046] (3) Deproteinization: dissolving the crude polysaccharide of Mortierella...

Embodiment 2

[0052] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculum amount of 5% (weight percentage), and culture it statically for 50 days at 15°C to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 15%, peptone 3%, cicada pupa powder 6%, KH 2 P O 4 0.6%, MgSO 4 .7H 2 O 0.1%, the balance is sterile water.

[0053] (2) Alcohol precipitation: Concentrate the fermented liquid of Mortierella sinensis in step (1) to obtain a concentrated liquid, add 5 times the volume of the concentrated liquid, and the volume percentage concentration is 96% ethanol aqueous solution, stir and mix, place in 2 Precipitate overnight in the refrigerator at ℃, then centrifuge at 5000rpm at 2℃ for 30min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis;

[0054] (3) Deproteinization: dissolving the crude polysaccharide of Mortierella sinensi...

Embodiment 3

[0060] (1) Submerged fermentation: Inoculate Mortierella sinensis species into the culture medium with an inoculation amount of 10% (percentage by weight), and culture it statically for 30 days at 22°C to obtain Mortierella sinensis fermentation broth; The culture medium contains (weight percentage): glucose 25%, peptone 7%, cicada pupa powder 12%, KH 2 PO 4 1.2%, MgSO 4 .7H 2 O 1%, the balance is sterile water.

[0061] (2) Alcohol precipitation: Concentrate the Mortierella sinensis fermentation liquid in step (1) to obtain a concentrated liquid, add 4 times the volume of the concentrated liquid, and the volume percentage concentration is 90% ethanol aqueous solution, stir and mix, place in 5 Precipitate overnight in the refrigerator at ℃, then centrifuge at 4000rpm at 5℃ for 30min, take the precipitate obtained by centrifugation to obtain the crude polysaccharide of Mortierella sinensis once;

[0062] (3) Deproteinization: dissolving the crude polysaccharide of Mortiere...

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Abstract

The invention discloses a Chinese cordyceps sinensis protein polysaccharide and preparation and application thereof. The Chinese cordyceps sinensis protein polysaccharide is composed of polysaccharide with 55%-60% weight percentage and protein with 40%-45% weight percentage; and the ingredients of the polysaccharide are mannose, ribose, glucose, galactose, xylose and arabinose; the ratio of amount of substances among the mannose, the ribose, the glucose, the galactose, the xylose and the arabinose is 6.42-6.51: 2.20-2.32: 65.85-65.95: 2.10-2.18: 4.20-4.30. The Chinese cordyceps sinensis protein polysaccharide preparation method comprises the following steps: extracting the Chinese cordyceps sinensis protein polysaccharide by aqueous extract and alcohol precipitation, removing the protein, dialyzing, exchanging chromatography by anion and purifying gel filter layer chromatography through an enzyme-Sevage combination method, and freezing, drying and separating purification components in vacuum, and the Chinese cordyceps sinensis protein polysaccharide is acquired. According to the preparation and the application thereof, proximate analysis, structural identification and immunologic function study are carried out on the components of polysaccharide after purification, and the facts that the Chinese cordyceps sinensis protein polysaccharide has strong immunoregulation activity and is a potential immunological enhancement substance are discovered.

Description

technical field [0001] The invention relates to the field of fungal exopolysaccharides, in particular to a Chinese Mortierella proteoglycan and its preparation and use, and belongs to the field of macromolecule and molecular biology. Background technique [0002] Together with ginseng and deer antler, Cordyceps sinensis is listed as one of the three major tonics in China. It is a traditional Chinese medicinal material that is precious and nourishing. The larvae are made rigid, and under suitable conditions, a long rod-shaped sub-seat is formed from the head of the dead larvae in summer. Hirsutella sinensis Liu, Guo, Yu, Zeng is the conidia stage of Cordyceps sinensis, once used Hirsutella hepialid Chen, Zhen (Liu Xijin et al., 1989, Isolation and identification of the asexual stage of Cordyceps sinensis , Acta Mycologica, 8(1):35-40). [0003] Wild Cordyceps sinensis is scarce due to the impact of special ecological environment, and now most people use solid or liquid subm...

Claims

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Application Information

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IPC IPC(8): C08B37/00A01G1/04A61P37/02
Inventor 贺亮程俊文李海波李卫旗魏海龙付立忠胡传久
Owner ZHEJIANG FORESTRY ACAD
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