Bacteria fermenting and producing L-lysine method by using changed aconitase to regulate and control unit

Abstract

The invention provides a method of fermenting and producing L-lysine. The bacteria fermenting and producing L-lysine method by using changed aconitase to regulate and control a unit comprises the following steps: transforming the regulate and control unit of the wild type of acnA gene on bacteria chromosomes, enabling the expression quantity of the aconitase to lower and not to disappear; and using the transformed bacteria to ferment and to produce the L-lysine. Additionally, the invention further provides a method and application derived from the bacteria fermenting and producing L-lysine method by using changed aconitase to regulate and control the unit, and the bacteria, and the like which can be used in the method and the application.

Description

technical field [0001] The present invention belongs to the field of amino acid fermentation, and in particular, the present invention relates to a method for producing L-lysine by fermentation, a method and application of its derivation, and bacteria that can be used in these methods and applications. Background technique [0002] Production of L-lysine by fermentation of L-lysine-producing bacteria (eg, Escherichia coli of the genus Escherichia and rod-shaped bacteria of the genus Corynebacterium) has been industrially used. These bacteria can be bacteria isolated from nature, bacteria obtained through mutagenesis or genetic engineering, or both. In the current literature reports, the attention of genetic engineering transformation is mainly focused on genes such as pnt, dap and ppc, and there is no focus on encoding aconitase (such as aconitase A) for L-lysine production regulatory elements of genes. [0003] Aconitase is an enzyme in the tricarboxylic acid cycle that c...

AI Technical Summary

Problems solved by technology

It is currently known that in Escherichia coli, the acnA gene (its nucleotide sequence is shown in SEQ ID No: 1) encodes aconitase A, but it may be because its metabolism is too far away from the final L-lysine product, There are too many and complex branches of intermediate metabolism, but it has not attracted people's attention in L-lysine fermentation

[0004] After long-term research and practice, especially with some luck, the inventor accidentally found that the transformation of the regulatory elements of the acnA gene can help improve the production of L-lysine Yield; however, the prior art either introduces beneficial enzyme genes with improved expression and/or enzyme activity by increasing copies and/or site-directed mutations, or

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