Check patentability & draft patents in minutes with Patsnap Eureka AI!

Propagating method of hemiboea rubribracteata

A technology for the concentration and concentration of sphaerocarpium cannabis, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of high environmental condition requirements, harsh habitats, narrow distribution range and the like, and achieves a wide range of application prospects. Effect

Active Publication Date: 2013-06-12
INST OF BOTANY CHINESE ACAD OF SCI
View PDF3 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hemiboea cavaleriei is a genus endemic to China, with a total of 24 species and 5 varieties, including Guizhou Hemiboea cavaleriei, Emei Hemiboea omeiensis, Hemiboea subcapitata, short stem Hemiboea subacaulis and South China Hemiboea follicularis are traditional folk herbal medicines, but due to their narrow distribution range, high requirements on environmental conditions, and harsh habitats, the populations are too small, and in nature Under normal conditions, the plants of this genus often reproduce vegetatively on stolons, and the propagation speed is relatively slow.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Propagating method of hemiboea rubribracteata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the propagation of red bud semicapsule lettuce

[0039] 1. Preparation of explants (operated in ultra-clean bench)

[0040] Take the leaves of Porphyria spp., wash them with tap water, soak them in 0.1% (mass ratio) HgCl2 aqueous solution for 2 minutes, wash them with sterile water for 4 times, and then cut the leaves into 0.8cm 2 The size is the explant.

[0041] 2. Induction of adventitious buds

[0042] The explants obtained in step 1 were inoculated on the induction medium, and cultured in the dark at 25±1°C for 50 days (adventitious buds formed successively from the 20th day).

[0043] Induction medium (pH 6.0) was obtained by adding sucrose, KT and IBA to MS solid medium, and the concentration of sucrose was 30g L -1 , The concentration of KT is 1.0mg·L -1 , The concentration of IBA is 0.5mg·L -1 .

[0044] 3. Proliferation and subculture

[0045] Transfer the adventitious buds obtained in step 2 to the propagation medium, 25±1°C, and light fo...

Embodiment 2

[0052] Embodiment 2, the propagation of red bud semicapsule lettuce

[0053] 1. Preparation of explants (operated in ultra-clean bench)

[0054] Get the blades of the Radix Scutellaria semicapsules, wash it with tap water, and then use 0.1% (mass ratio) HgCl 2 Soak in aqueous solution for 2 minutes, wash 4 times with sterile water, and then cut the leaves into 0.8cm 2 The size is the explant.

[0055] 2. Induction of adventitious buds

[0056] The explants obtained in step 1 were inoculated on the induction medium, and the light was 14 hours a day at 23±1°C (the light intensity was 10 μmol·m -2 ·s -1 ) for 45 days (in which adventitious buds gradually formed from the 20th day).

[0057] Induction medium (pH 5.8) was obtained by adding sucrose, KT and IBA to MS solid medium, the concentration of sucrose was 30g L -1 , The concentration of KT is 0.2mg·L -1 , The concentration of IBA is 0.2mg·L -1 .

[0058] 3. Proliferation and subculture

[0059] Transfer the adventit...

Embodiment 3

[0066] Embodiment 3, the propagation of red bud semicapsule lettuce

[0067] 1. Preparation of explants (operated in ultra-clean bench)

[0068] Get the blades of the Radix Scutellaria semicapsules, wash it with tap water, and then use 0.1% (mass ratio) HgCl 2 Soak in aqueous solution for 2.5 minutes, wash 4 times with sterile water, and then cut the leaves into 0.8cm 2 The size is the explant.

[0069] 2. Induction of adventitious buds

[0070] The explants obtained in step 1 were inoculated on the induction medium, 27 ± 1 ° C, 14 hours of light per day (the light intensity was 20 μmol m -2 ·s -1 ) for 40 days (in which adventitious buds gradually formed from the 20th day).

[0071] Induction medium (pH 5.8) was obtained by adding sucrose, KT and IBA to MS solid medium, the concentration of sucrose was 30g L -1 , The concentration of KT is 0.5mg·L -1 , The concentration of IBA is 0.3mg·L -1 .

[0072] 3. Proliferation and subculture

[0073] Transfer the adventitiou...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a propagating method of hemiboea rubribracteata. The propagating method comprises the following steps of: (1) sterilizing leaves of the hemiboea rubribracteata and chopping the leaves into blocks to obtain explants; (2) inoculating the explants obtained in step (1) to an induction medium, cultivating the explants at 20-28 DEG C and at light intensity below 20 micromole.m<-2>.s<-1> to obtain adventitious buds; (3) transferring the adventitious buds obtained in step (2) to a propagation medium and cultivating the adventitious buds with illumination at 20-28 DEG C to obtain clustered shoots; and (4) segmenting the clustered shoots obtained in step (3), transferring the segmented shoots to a rooting medium, and cultivating the shoots with illumination at 20-28 DEG C until the shoots take root. The propagating method serves as a way for propagating hemiboea rubribracteata manually and quickly, makes scale production of hemiboea rubribracteata possible, lays a good foundation for further protecting and using the species in the future, and has broad application prospect.

Description

technical field [0001] The present invention relates to a method for propagating the genus syringa. Background technique [0002] There are about 150 genera and more than 3,700 species of Gesneriaceae plants in the world, distributed in tropical to temperate regions from eastern and southern Asia, Africa, southern Europe, Oceania, South America to Mexico. There are 58 genera and 463 species known in my country (27 genera are native to China), all of which belong to the Gesneriae subfamily, most of which have high ornamental value, among which there are more than 100 kinds of medicinal plants, and a considerable part for endangered plants. [0003] Hemiboea cavaleriei is a genus endemic to China, with a total of 24 species and 5 varieties, including Guizhou Hemiboea cavaleriei, Emei Hemiboea omeiensis, Hemiboea subcapitata, short stem Hemiboea subacaulis and South China Hemiboea follicularis are traditional folk herbal medicines, but due to their narrow distribution range, h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 石雷贾蕙宁陈维伦郭东红邢全李扬
Owner INST OF BOTANY CHINESE ACAD OF SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com