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A kind of polypeptide pro-infection agent

A reagent and sequence technology, applied in the fields of peptides, genetic material components, gene therapy, etc., can solve the problem of low infection efficiency of lentiviral vector system

Active Publication Date: 2017-07-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of low infection efficiency of the retroviral vector system and the lentiviral vector system in the prior art, the present invention provides a reagent for promoting virus infection, which includes a polypeptide sequence, and the polypeptide sequence is an enveloped virus Derived polypeptide sequences of transmembrane proteins near the extramembrane region

Method used

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  • A kind of polypeptide pro-infection agent
  • A kind of polypeptide pro-infection agent
  • A kind of polypeptide pro-infection agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Peptide Design

[0020] Table 1

[0021]

[0022] Characterization of pro-infectious activity

[0023] figure 1 Comparison of pro-infection activities of MPER, NK13 and QW13.

Embodiment 2

[0025] Peptide Design

[0026] Table 2

[0027] name describe sequence sequence listing NK13 C-terminal polypeptide of MPER region Ac-NWFDITNWLWYIK SEQ ID NO:1 NK16 NK13-derived peptides Ac-NWFDITNWLWYIKKKK SEQ ID NO:2

[0028] Characterization of pro-infectious activity

[0029] figure 2 Comparison of pro-infection activities of NK16 and NK13.

[0030] Compared with the activity of commonly used pro-infection reagents

[0031] image 3 Comparison of the pro-infection activity of NK16 and DEAE dextran at different infection times.

[0032] Application to other enveloped virus infections

[0033] Figure 4 GFP fluorescence flow cytometric analysis of Hela cells infected with FIV / VSV-G.

Embodiment 3

[0035] Figure 5 Pro-infection activity of NK16 under different ionic strength conditions.

[0036] Image 6 Pro-infection activity of NK16 at different pH conditions.

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Abstract

The invention relates to a polypeptide with HIV infection promoting ability. The HIV infection promoting polypeptide comprises a peptide sequence, containing a terminal C peptide of near extramembrane fragment MPER fragment of an HIV-1 envelope protein GP41. The polypeptide infection promoting agent provided by the invention has significant activity in promoting envelope viral infection, and shows activity superior to that of a common infection promoting agent DEAE at a cellular level. Therefore, the polypeptide infection promoting agent plays an important role in the development of retroviral or lentiviral gene therapy.

Description

technical field [0001] The invention relates to a polypeptide capable of promoting HIV infection, and the polypeptide sequence is derived from the membrane-proximal outer region MPER of HIV envelope protein GP41. Background technique [0002] At present, the target gene transfer tools in gene therapy are divided into two categories: viral vectors and non-viral vectors. The vector system based on viral vectors such as retroviruses and adenoviruses is the most commonly used. The advantage of retroviral vectors is that the transfection range is wide, and the transferred foreign gene can be integrated into the host cell genome; the disadvantage is that it can only be integrated into the dividing cell; the insertable foreign gene fragment is small, and it is difficult to meet the insertion of larger genes; There is a possibility of generating wild-type virus or helper virus, and random integration may have adverse effects. The emergence of lentiviral vectors has improved these p...

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K48/00
Inventor 单亚明孔维张丽双张浩管珊珊
Owner JILIN UNIV
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