Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof

A technology of fingerprints and determination methods, which is applied in the field of traditional Chinese medicine fingerprint analysis, can solve problems such as poor resolution of fingerprints, and achieve the effects of good absorption value, high similarity, and easy identification

Active Publication Date: 2013-06-12
贵州汉方制药有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the patent document with the announcement number "CN101327246B" and the title "Fingerprint Detection Method and Standard Fingerprint of Astragalus medicinal materials, intermediates and their preparations", 80%-95% methanol is

Method used

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  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof
  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof
  • Measurement method of radix astragali granule fingerprint and characteristic fingerprint thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1 of the present invention: The method for measuring the fingerprint of Huangqi granules includes the following steps:

[0048] (1) Preparation of test solution: pulverize astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0 g of the powder, add 10 mL of 10% methanol solution to dissolve, extract with ultrasound for 5 minutes, then cool to make up for the weight loss, shake, and filter Once, take the subsequent filtrate and pass it through a 0.45μm microporous membrane to obtain the test solution;

[0049] (2) Preparation of the reference solution: take the verbasin, formononetin, verbasin, and formononetin reference substances dried under reduced pressure to constant weight, and add methanol to each to make 0.562mg verbasin per 1mL. Glycoside, 0.254mg formononetin, 0.357mg verbasin, 0.274mg formononetin reference solution (stored in the refrigerator at 4℃ for later use);

[0050] (3) Determination: Chromatographic conditions: Chromatographic col...

Embodiment 2

[0052] Embodiment 2 of the present invention: The method for determining the fingerprint of Huangqi granules includes the following steps:

[0053] (1) Preparation of test solution: pulverize astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0g of the powder, add 10 mL of 10% methanol solution to dissolve, extract with ultrasound for 10 minutes, then cool to make up for the weight loss, shake, and filter Once, take the subsequent filtrate and pass it through a 0.45μm microporous membrane to obtain the test solution;

[0054] (2) Preparation of reference solution: take the verbasin, formononetin, verbasin, and formononetin reference substance dried under reduced pressure to constant weight, and add methanol to each to make 0.562mg verbasin per 1mL. Glycoside, 0.254mg formononetin, 0.357mg verbasin, 0.274mg formononetin reference solution (stored in the refrigerator at 4℃ for later use);

[0055] (3) Determination: Chromatographic conditions: Chromatographic column...

Embodiment 3

[0057] Embodiment 3 of the present invention: The method for measuring the fingerprint of Huangqi granules includes the following steps:

[0058] (1) Preparation of test solution: pulverize astragalus granules, pass through a 60-mesh sieve, accurately weigh 1.0g of the powder, add 10 mL of 10% methanol solution to dissolve, and extract twice with ultrasonic for 30 minutes each time, then cool to make up for the weight loss , Shake well, filter, take the subsequent filtrate and pass it through a 0.45μm microporous membrane to obtain the test solution;

[0059] (2) Preparation of the reference solution: take the verbasin, formononetin, verbasin, and formononetin reference substances dried to constant weight under reduced pressure, and add methanol to each to make 0.562mg verbasin per 1mL. The reference solution of glycosides, 0.254mg formononetin, 0.357mg verbasin and 0.274mg formononetin;

[0060] (3) Determination: Chromatographic conditions: Chromatographic column is Agilent ZORBAX...

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Abstract

The invention discloses a measurement method of a radix astragali granule fingerprint. The measurement method of the radix astragali granule fingerprint comprises the steps of preparation of a test solution, preparation of a comparison product solution, high performance liquid chromatograph measurement, and treatment of data and fingerprint. The invention further discloses the radix astragali granule fingerprint obtained by the method. Compared with the prior art, the radix astragali granule fingerprint obtained by the invention has the characteristics of more peaks, good peak form, good peak separation effect, larger peak area, easiness of verification, high similarity, accuracy and reliability. The method provided by the invention is simple and convenient, fast, accurate, stable and reliable, can be used for the quality control of a radix astragali granule sample, can be used for comprehensively, objectively and scientifically evaluating the quality of radix astragali granules, and provides the foundation and the reference for the rational drug use and further drug efficacy study of the radix astragali granules.

Description

Technical field [0001] The present invention relates to a traditional Chinese medicine fingerprint analysis method, in particular to a method for determining the HPLC characteristic fingerprint of Huangqi granules and the characteristic fingerprint of Huangqi granules obtained by the method. Background technique [0002] Most of the traditional Chinese medicines and their preparations in my country lack strict quality standards and scientific testing methods, it is difficult to effectively control their internal quality, cannot guarantee the safety and effectiveness of the medicines, and do not meet the requirements of the international pharmaceutical market, which seriously restricts the Chinese medicine industry development of. Strengthening the research on the quality control of Chinese herbal medicines is a key issue in the standardization and standardization of Chinese medicines. Only scientific quality control of Chinese medicinal materials can ensure the quality of Chinese...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 周欣陈华国赵超龚小见赵杨邓杰
Owner 贵州汉方制药有限公司
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