Method for producing food material with high GABA function with rice endogenous enzyme activation method

An endogenous, rice-based technology, applied in the fields of biochemical equipment and methods, food preparation, enzymes, etc., can solve the problems that are still in its infancy, and achieve the effect of improving brain function and liver function.

Inactive Publication Date: 2015-01-28
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2007, Japan's Coca-Cola Company launched the GABA functional drink "AQUARIUS SHARP CHARGE" with relaxation and anti-stress effects for the public. develop and improve

Method used

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  • Method for producing food material with high GABA function with rice endogenous enzyme activation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared by rice hulling and removing impurities) and put it in a shallow dish. Add 4 times the volume of water and soak at 25°C for 3h. Spread the soaked brown rice evenly in a shallow pan covered with four layers of gauze, add an appropriate amount of water, cover with wet gauze, germinate at 25°C for 24 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue grinder, take a certain amount of brown rice pulp, add 8.0mmol / L substrate L-glutamic acid, 0.2mmol / L coenzyme PLP, 0.2 mmol / L Activator CaCl 2 , converted to GABA in PBS buffer with pH 6.1, and the conversion temperature was controlled at 37.5°C.

[0024] (2) GAD (glutamic acid decarboxylase) extraction: Weigh 100g of germinated brown rice, add 50mmol / L phosphate buffer (pH5.7), 0.5mmol / L vitamin B 6 , 2mmol / L EDTA, 0.2% β-mercaptoethanol, ...

Embodiment 2

[0027] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared by rice hulling and removing impurities) and put it in a shallow dish. Add 4 times the volume of water and soak at 30°C for 6h. Spread the soaked brown rice evenly in a shallow pan covered with four layers of gauze, add an appropriate amount of water, cover with wet gauze, germinate at 30°C for 36 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue grinder, take a certain amount of brown rice pulp, add 8.5mmol / L substrate sodium glutamate, coenzyme PLP, 0.2mmol / L activator CaCl 2 , converted into GABA in PBS buffer solution with pH 6.2, and the conversion temperature was controlled at 38.0°C.

[0028] (2) GAD (glutamic acid decarboxylase) extraction: Weigh 200g of brown rice, add 800ml of extraction solution (50mmol / L potassium phosphate buffer, pH5.6, 2mmol / L mercaptoethanol, 2mmol / L E...

Embodiment 3

[0031] (1) Preparation of GABA (γ-aminobutyric acid): Weigh 200g of brown rice (brown rice is prepared by rice hulling and removing impurities) and put it in a shallow dish. Add 4 times the volume of water and soak at 35°C for 9h. Spread the soaked brown rice evenly in a shallow pan covered with four layers of gauze, add an appropriate amount of water, cover with wet gauze, germinate at 30°C for 46 hours and spray water regularly. Weigh 50g of germinated brown rice, add 200mL of water and grind it into a slurry with a high-speed tissue grinder, take a certain amount of brown rice pulp, add 9.0mmol / L substrate sodium glutamate, coenzyme PLP, 0.2mmol / L activator CaCl 2 , at pH 6.3 in PBS buffer (containing 50mmol / L phosphate buffer (pH5.7), 0.2mmol / L pyridoxal phosphate (PLP), 2mmol / L EDTA, 0.2% β-mercaptoethanol, 0.15mol / L NaCl) to generate GABA, and control the conversion temperature to 38.5°C.

[0032] (2) GAD (glutamic acid decarboxylase) extraction: Weigh 100g of germi...

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Abstract

The invention discloses a method for producing a food material with a high GABA (gamma amino acid butyric acid) function with a rice endogenous enzyme activation method. A glutamic acid decarboxylase activation technology is adopted on the basis that rice endogenous glutamate decarboxylase characteristics and an activity regulatory mechanism are defined, so that healthy rice food with a high GABA content is produced; germinated brown rice rich in GABA is taken as a raw material, and with the adoption pretreatment of aminotransferase for passivating and decomposing GABA, germ-remained GABA polished rice is produced; a natural nutrient solution containing a glutamic acid decarboxylase activating agent and a special buffer system is utilized for sprouting; and a rice husk and a rice germ are taken as a GAD (glutamic acid decarboxylase) source, a food ingredient rich in GABA is produced after the GAD source is immobilized, and the food ingredient is applied to GABA nutrition coordination rice powder production. The method is simple and convenient, development of food materials rich in GABA functional factors can be accelerated, and the food material can be used as or for functional food which is resistant to hypertension and can promote brain functions and liver functions.

Description

technical field [0001] The invention relates to the field of food, in particular to a method for producing food materials with high GABA function by using rice endogenous enzyme activation method, so as to improve the structure and nutritional structure of food products. Background technique [0002] As a bioactive factor with multiple physiological functions, GABA has been widely used in nutritional supplements and functional foods in the United States, Japan and other countries. In 1996, GABA-rich rice germ products were commercialized in Japan. Japanese scientists use rice germ and other raw materials to develop and manufacture GABA-rich functional food ingredients, and apply them to beverages, jams, pastries, biscuits, seasonings and other products. Existing reports show that high-concentration GABA beverages produced by applying biotechnology and GABA-rich food ingredients produced by lactic acid bacteria can be directly used or used as functional foods for antihyperten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C12N9/88A23L1/015A23L1/185A23L1/29A23L7/104A23L5/20A23L7/20A23L33/00
Inventor 王立峰陈静宜
Owner NANJING UNIV OF FINANCE & ECONOMICS
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