Rapid detection kit for vibrio parahaemolyticus and detection method thereof

Abstract

The invention provides a rapid detection kit for vibrio parahaemolyticus and a detection method thereof. The time spent on sample solution preparation and result judge consumes 50min and the detection sensitivity can reach 100fg / 25g. The rapid detection kit and the detection method have the advantages of being simple and convenient in operation process, short in consumed time, strong specificity, high sensitivity, low cost and the like. The method can effectively analyze a carrier state of a sample and can treat the sample by high throughput; the labor is greatly saved and the detection cost is reduced; and the method can be widely applied to the detection of the vibrio parahaemolyticus of the samples in aquatic products, gastrointestinal vomits and excrements of clinical patients and the like by all grades of supervision and management mechanisms of foods and drugs, disease prevention and control laboratories and the like.

Description

technical field [0001] The invention belongs to the field of molecular biology detection, in particular to a rapid detection kit for Vibrio parahaemolyticus and its detection method, which can be applied to food and drug supervision and management agencies at all levels, entry-exit inspection and quarantine agencies, disease prevention and control agencies, etc. Detection of Vibrio parahaemolyticus in aquatic food and rapid detection of food poisoning incidents caused by Vibrio parahaemolyticus. Background technique [0002] Vibrio parahaemolyticus (Vibrio parahaemolyticus, Vp) is a halophilic Gram-negative non-spore bacterium, mainly distributed in offshore seawater, seabed sediments, sea-river junctions, seafood and salted food, river water and A large number of Vibrio parahaemolyticus can also be detected in freshwater products such as fish, shrimp and crab. Vibrio parahaemolyticus mainly causes acute gastroenteritis and sepsis with acute diarrhea, vomiting and fever as ...

AI Technical Summary

Problems solved by technology

According to relevant literature reports, some researchers have applied this technology to the detection of parahaemolytic, but the reaction time is long, 45-60 minutes to complete the detection, and the specificity of the primers is poor, there are false positive and false negative results

[0007] In the invention with the patent number CN 102586452 A, immunomagnetic beads are used for pretreatment to enrich bacteria, and circular primers are added to the reaction system to speed up the reaction. Although false negative and false positive results are greatly avoided, the price of immunomagnetic beads Expensive, the entire detection work takes nearly 2 hours, it is difficult to popularize in daily work