Application of rhamnolipid as biological pesticide and biological insecticide

A rhamnolipid and biopesticide technology, which is applied in the direction of insecticides, biocides, and chemicals for biological control, etc., can solve the problems of heavy processing workload, unfavorable human health and environmental protection, and difficulty in sedimentation , to reduce the harm to the human body and the environment, inhibit plant fungal diseases, and reduce the amount of sludge treatment

Active Publication Date: 2015-01-07
浙江圣达紫金生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most conventional insecticides are highly toxic, and frequent use is not conducive to human health and environmental protection. As a biological insecticide, rhamnolipid has a significant insecticidal effect. Reports about pesticides
[0009] In addition, the organic matter in the oily sludge of petrochemical enterprises is complex and has different components, which has an unpleasant smell and is easy to pollute the environment. It is easy to form a suspended emulsion with various impurities such as sediment, which has a high viscosity and is difficult to settle, resulting in a huge processing workload.
Existing wastewater and sludge treatment methods cannot effectively treat oily sludge, causing serious pollution to the environment

Method used

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  • Application of rhamnolipid as biological pesticide and biological insecticide
  • Application of rhamnolipid as biological pesticide and biological insecticide
  • Application of rhamnolipid as biological pesticide and biological insecticide

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] The preparation method of this rhamnolipid, concrete steps are as follows:

[0026] 1. Strain cultivation: Inoculate the Pseudomonas aeruginosa ZJU-211 strain into the shake flask seed medium for cultivation, the temperature is 32-38°C, the shaker speed is 120-200rpm, and cultivate for 24-48 hours to obtain the shake flask strain.

[0027] The composition of the shake flask seed medium (g / L): waste oil, 30-40: NaNO 3 , 2~4; NaCl, 0.5~1.5; KCl, 0.5~1.5; CaCl 2 2H 2 O, 0.05~0.2; KH 2 PO 4 , 2~4; Na 2 HPO 4 12H 2 O, 2~4; MgSO 4 , 0.1~0.3; trace elements, 1.5~2.5mL / L, the balance is water.

[0028] Trace element composition (g / L): FeCl 3 ·6H 2 O 0.01~0.08, ZnSO 4 ·7H 2 O 0.1~0.75, CuSO 4 ·5H 2 O 0.01~0.075, MnSO 4 ·H 2 O 0.1~0.75, H 3 BO 3 , 0.01 ~ 0.15, the balance is water.

[0029] 2. Fermentation: Inoculate the shake flask strain into a 10L or integral multiple fermenter, and cultivate it with the fermentation medium. The inoculation amount is 3-10% (...

Embodiment 1

[0043] Embodiment 1: the preparation of debacterial rhamnolipid

[0044] 1. Strain: The fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosa ZJU-211)

[0045] 2. The strain was inoculated in the seed medium at a temperature of 35° C., the rotation speed of the shaker was 180 rpm, and cultured for 48 hours to obtain the shake flask strain.

[0046] The composition of the seed medium is as follows (g / L):

[0047] McDonald's Waste Grease, 30: NaNO 3 , 2; NaCl, 0.5; KCl, 0.5; CaCl 2 2H 2 O, 0.05; KH 2 PO 4 , 2; Na 2 HPO 4 12H 2 O,2; MgSO 4 , 0.1; trace elements, 1.5mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.01, ZnSO 4 ·7H 2 O 0.1, CuSO 4 ·5H 2 O 0.01, MnSO 4 ·H 2 O 0.1g / L, H 3 BO 3 , 0.01, and the balance is water.

[0048] 3. Production of rhamnolipid by fermentation in 10L fermenter

[0049] The shake flask strain was inoculated into a 10L fermenter with an inoculum volume of 5% (...

Embodiment 2

[0054] Embodiment 2: the preparation of rhamnolipid concentrate

[0055] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosa ZJU-211)

[0056] 2. The strain was inoculated in the seed medium at a temperature of 38° C., the rotation speed of the shaker was 200 rpm, and cultured for 48 hours to obtain the shake flask strain.

[0057] The composition of the seed medium is as follows:

[0058] Rapeseed oil processing leftovers, 40g / L: NaNO 3 , 4g / L; NaCl, 1.5g / L; KCl, 1.5g / L; CaCl 2 2H 2 O, 0.2g / L; KHxPO 4 , 4g / L; Na 2 HPO 4 12H 2 O, 4g / L; MgSO 4 , 0.3g / L; trace elements, 2.5mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.08g / L, ZnSO 4 ·7H 2 O 0.75g / L, CuSO 4 ·5H 2 O 0.075g / L, MnSO 4 ·H 2 O 0.75g / L, H 3 BO 3 , 0.15g / L, and the balance is water.

[0059] 3. Production of rhamnolipid by fermentation in 50L fermenter

[0060] The shake flask strain was inoculated into a 50L f...

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Abstract

The invention discloses application of rhamnolipid as a biological pesticide and a biological insecticide. A solution containing 20 to 500 mg / L of rhamnolipid has an inhibition rate of 40 to 90% on mycelial growth and spore germination of a plant pathogenic fungus when used for treating the plant pathogenic fungus, so the solution can effectively inhibit plant fungal diseases. And the solution containing 20 to 500 mg / L of rhamnolipid has striking insecticidal effects on cockroach and aphid.

Description

[0001] This application is a divisional application of a patent application with an application date of March 30, 2010, an application number of 201010136140.3, and an invention title of "A Preparation Method and Application of Rhamnolipid". technical field [0002] The invention relates to the technical field of biosurfactants, in particular to the application of a rhamnolipid as a biopesticide and a biopesticide. Background technique [0003] Biosurfactants are a class of surfactants produced by microorganisms, animals or plants, which are very similar in performance to chemically synthesized surfactants, that is, the molecules are composed of polar hydrophilic groups and non-polar lipophilic groups. A molecular layer is formed on the phase interface to reduce the energy of the interface, and it has good surface activity, such as emulsification, dispersion, wetting and penetration. Compared with chemically synthesized surfactants, biosurfactants have stronger surface activ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P3/00A01P7/04
Inventor 孟琴张国亮
Owner 浙江圣达紫金生物科技有限公司
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