Application of rhamnolipid as biological pesticide and biological insecticide
A rhamnolipid and biopesticide technology, which is applied in the direction of insecticides, biocides, and chemicals for biological control, etc., can solve the problems of heavy processing workload, unfavorable human health and environmental protection, and difficulty in sedimentation , to reduce the harm to the human body and the environment, inhibit plant fungal diseases, and reduce the amount of sludge treatment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0025] The preparation method of this rhamnolipid, concrete steps are as follows:
[0026] 1. Strain cultivation: Inoculate the Pseudomonas aeruginosa ZJU-211 strain into the shake flask seed medium for cultivation, the temperature is 32-38°C, the shaker speed is 120-200rpm, and cultivate for 24-48 hours to obtain the shake flask strain.
[0027] The composition of the shake flask seed medium (g / L): waste oil, 30-40: NaNO 3 , 2~4; NaCl, 0.5~1.5; KCl, 0.5~1.5; CaCl 2 2H 2 O, 0.05~0.2; KH 2 PO 4 , 2~4; Na 2 HPO 4 12H 2 O, 2~4; MgSO 4 , 0.1~0.3; trace elements, 1.5~2.5mL / L, the balance is water.
[0028] Trace element composition (g / L): FeCl 3 ·6H 2 O 0.01~0.08, ZnSO 4 ·7H 2 O 0.1~0.75, CuSO 4 ·5H 2 O 0.01~0.075, MnSO 4 ·H 2 O 0.1~0.75, H 3 BO 3 , 0.01 ~ 0.15, the balance is water.
[0029] 2. Fermentation: Inoculate the shake flask strain into a 10L or integral multiple fermenter, and cultivate it with the fermentation medium. The inoculation amount is 3-10% (...
Embodiment 1
[0043] Embodiment 1: the preparation of debacterial rhamnolipid
[0044] 1. Strain: The fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosa ZJU-211)
[0045] 2. The strain was inoculated in the seed medium at a temperature of 35° C., the rotation speed of the shaker was 180 rpm, and cultured for 48 hours to obtain the shake flask strain.
[0046] The composition of the seed medium is as follows (g / L):
[0047] McDonald's Waste Grease, 30: NaNO 3 , 2; NaCl, 0.5; KCl, 0.5; CaCl 2 2H 2 O, 0.05; KH 2 PO 4 , 2; Na 2 HPO 4 12H 2 O,2; MgSO 4 , 0.1; trace elements, 1.5mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.01, ZnSO 4 ·7H 2 O 0.1, CuSO 4 ·5H 2 O 0.01, MnSO 4 ·H 2 O 0.1g / L, H 3 BO 3 , 0.01, and the balance is water.
[0048] 3. Production of rhamnolipid by fermentation in 10L fermenter
[0049] The shake flask strain was inoculated into a 10L fermenter with an inoculum volume of 5% (...
Embodiment 2
[0054] Embodiment 2: the preparation of rhamnolipid concentrate
[0055] 1. Strain: the fermentation strain is Pseudomonas aeruginosa ZJU-211 (Pseudomonas aeruginosa ZJU-211)
[0056] 2. The strain was inoculated in the seed medium at a temperature of 38° C., the rotation speed of the shaker was 200 rpm, and cultured for 48 hours to obtain the shake flask strain.
[0057] The composition of the seed medium is as follows:
[0058] Rapeseed oil processing leftovers, 40g / L: NaNO 3 , 4g / L; NaCl, 1.5g / L; KCl, 1.5g / L; CaCl 2 2H 2 O, 0.2g / L; KHxPO 4 , 4g / L; Na 2 HPO 4 12H 2 O, 4g / L; MgSO 4 , 0.3g / L; trace elements, 2.5mL / L, the balance is water. Described trace element composition is as follows: FeCl 3 ·6H 2 O 0.08g / L, ZnSO 4 ·7H 2 O 0.75g / L, CuSO 4 ·5H 2 O 0.075g / L, MnSO 4 ·H 2 O 0.75g / L, H 3 BO 3 , 0.15g / L, and the balance is water.
[0059] 3. Production of rhamnolipid by fermentation in 50L fermenter
[0060] The shake flask strain was inoculated into a 50L f...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com