Method for cultivating hybridized mouse with Myd88 molecule selectively-knocked-out dendritic cells

Abstract

The invention discloses a method for cultivating a hybridized mouse with Myd88 molecule selectively-knocked-out dendritic cells. The method comprises the following steps of (1) purchasing a CDllc-cre (a tool mouse with dendritic cells) and a conditional Myd88 molecule knocked-out mouse from an American Jackson company; (2) hybridizing the two mice; (3) establishing a primer to perform genotype identification to hybridized mouse descendants and performing screening to obtain the hybridized mouse (myd88Flox / Flox; Cdllc-cre) with the Myd88 molecule selectively-knocked-out dendritic cells. The method has the advantages that a biological characteristic of the hybridized mouse with the Myd88 molecule selectively-knocked-out dendritic cells and cultivated by means of the method is a whole-body dendritic cell non-expression Myd88 molecule, and the hybridized mouse can be well applied to viviperception of the dendritic cell immunological effect and a molecular mechanism.

Description

technical field [0001] The invention relates to the field of cell construction, and relates to a method for constructing a hybrid mouse that selectively knocks out the Myd88 molecule in dendritic cells. Background technique [0002] Dendritic cells (DCs) have long been considered as a powerful and diverse antigen-presenting cell in the human body. The immune response can then achieve physiological defense or pathological aggravation of damage. [0003] The applicant has been engaged in DCs derived from the small intestine for a long time, because of its large number, direct contact with the largest bacterial pool in the human body-intestinal content, normal state of rest to achieve immune tolerance, and intestinal susceptibility to ischemia and hypoxia DCs derived from the small intestine have more complex and important characteristics. [0004] Intestinal mucosal lamina propria dendritic cells (lamina propria dendritic cells, LPDCs) are a group of antigen-presenting cells...

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Problems solved by technology

However, other in vivo studies have found that although intestinal LPDCs have increased exposure to bacterial flagellar antigens in emergency situations such as traumatic shock, they often exhibit functional inhibition.

Wang et al. found that under hypoxic conditions, intestinal-derived dendritic cells exhibited low maturity, increased secretion of TGF-β, decreased secretion of pro-inflammatory factors (IL-1β, IL-6, TNF-α), and could induce The state of immunosuppression dominated by the polarization of Treg cells; our previous research also found that the expression of CD80 / 86 and MHC II on the surface of LPDCs in severe traumatic shock mice was characterized by immature differentiation and decreased antigen presentation activity

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