Glutamyltranspeptidase for synthesizing gamma-polyglutamic acid and coding gene thereof

A glutamyl transpeptidase-based and glutamyl transpeptidase technology, which is applied in the field of glutamyl transpeptidase for synthesizing γ-polyglutamic acid and its encoding gene, and can solve the problem of glutamyl transpeptidase Enzymes and other problems, to achieve the effect of good application value
CN103232980BInactive Publication Date: 2015-01-28GUANGDONG IND TECHN COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG IND TECHN COLLEGE
Publication Date
2015-01-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides glutamyltranspeptidase for synthesizing gamma-polyglutamic acid and a coding gene thereof. The protein molecule of the glutamyltranspeptidase has the amino acid sequence as shown in SEQ ID NO: 2; and the gene of the glutamyltranspeptidase has the nucleotide sequence as shown in SEQ ID NO: 1. The invention also discloses a method for synthesizing the gamma-polyglutamic acid by utilizing recombinant glutamyltranspeptidase. By adopting the method, the gamma-polyglutamic acid is synthesized by utilizing the recombinant glutamyltranspeptidase and taking glutamine monomers as raw materials. The glutamyltranspeptidase can be used for synthesizing the gamma-polyglutamic acid by taking the glutamine monomers as the raw materials, thus having importance significance in enriching glutamyltranspeptidase family members and developing a new gamma-polyglutamic acid synthesis route.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering and enzyme engineering, in particular to a glutamyl transpeptidase for synthesizing γ-polyglutamic acid and its coding gene. Background technique

[0002] γ-polyglutamic acid (Poly-γ-glutamic acid, γ-PGA) is an anionic polymer composed of D- or L-glutamic acid through α-amino and γ-carboxyl to form a γ-amide bond . γ-PGA is a new water-soluble and biodegradable green biomaterial, which has the characteristics of edible, non-toxic, film-forming, cohesive, and moisturizing properties. Thickeners and humectants are used in medicine, food and cosmetics, and can also be used as water-retaining agents, antifreeze agents and fresh-keeping agents for fruits and vegetables, as well as flocculants and heavy metal chelating agents for water treatment. With the increasing awareness of environmental protection, γ-polyglutamic acid has attracted much attention as a biodegradable polymer material.

[0003] ...

Claims

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