PON-1 (paraoxonase-1) active detecting reagent kit and detecting method thereof

Abstract

The invention belongs to the technical field of clinical diagnosis of cardiovascular diseases, and particularly relates to a PON-1 (paraoxonase-1) active detecting reagent kit and a detecting method thereof which are suitable for quantitative assay of the activity of PON-1 in body fluid such as human serum in vitro through a biochemical analyzer and used as the assisting diagnosis. The reagent kit comprises a buffer solution and a substrate solution, wherein the buffer solution contains 0.15mol / L glycine, 2mol / L NaOH (sodium hydroxide), 2.5*10<-3>mol / L CaCl2 (calcium chloride) and a water solvent; and the substrate solution is 0.15mol / L to 0.42mol / L diethyl p-nitrophenyl phosphate, and the solvent is dimethyl sulfoxide. The PON-1 active detecting reagent kit has the advantages that the detecting method of the reagent kit is fast and simple, the early cardiovascular disease can be effectively diagnosed by assaying the activity of the PON-1 in the serum; and the synthesis method of the dimethyl sulfoxide is fast, simple and safe compared with the prior art, and the yield is high.

Description

technical field [0001] The invention belongs to the technical field of clinical diagnosis of cardiovascular diseases, and specifically relates to a PON-1 activity detection kit and a detection method using diethyl p-nitrophenyl phosphate as a substrate, which is suitable for quantitative determination of human body in vitro by a biochemical analyzer. The activity of PON-1 in serum and other body fluids is used for auxiliary diagnosis. Background technique [0002] Cardiovascular diseases, also known as circulatory system diseases, mainly include: heart disease, hypotension, hypertension, hyperglycemia, stroke, myocardial infarction, thrombosis, arteriosclerosis, etc., and its pathological basis is atherosclerosis. The prevention and early diagnosis of cardiovascular disease is one of the focuses of medical research in recent years. [0003] At present, it is often clinically determined by measuring serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cho...

AI Technical Summary

Problems solved by technology

However, there is an obvious shortcoming in this method: these index parameters can objectively reflect cardiovascular function only when the cardiovascular tissue is seriously damaged, and there is a possibility of misdiagnosis, while in early cardiovascular disease, these indicators cannot Timely reflection of subtle changes in cardiovascular function

However, since the color source released by paraoxon substrate hydrolysis is close to the color of serum, the measurement will affect the data, and the background needs to be deducted; on the other hand, in terms of measurement accuracy, sensitivity and application range, colorimetry is not as good as Ultraviolet spectrophotometry, which has a large error in the determination of low-activity PON-1, needs to increase the volume of serum samples to meet the detection requirements for sensitivity

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