Method for constructing crossbred mice which selectively knock out Myd88 molecules in intestinal epithelial cells

Abstract

The invention discloses a method for constructing crossbred mice which selectively knock out Myd88 molecules in intestinal epithelial cells. The method includes the following steps of purchasing CD11c-cre (intestinal epithelial cell tool mice) and conditioned Myd88 gene knocking out mice from the American Jackson company, crossbreeding the intestinal epithelial cell tool mice and the conditioned Myd88 gene knocking out mice, constructing a primer, performing genotype identification on progeny of the crossbreeding mice, and obtaining the crossbred mice (myd88Flox / Flox; Neurog3-cre) which selectively knock out the Myd88 molecules in the intestinal epithelial cells. The method for constructing the crossbred mice which selectively knock out the Myd88 molecules in the intestinal epithelial cells has the advantages that the biological characteristics of the constructed crossbred mice which selectively knock out the Myd88 molecules in the intestinal epithelial cells are unexpressed Myd88 molecules in the intestinal epithelial cells, and the constructed crossbred mice which selectively knock out the Myd88 molecules in the intestinal epithelial cells can be well applied to viviperception of intestine epithelium immunological effect and molecular mechanisms.

Description

technical field [0001] The invention relates to the field of cell construction, and relates to a method for constructing a hybrid mouse for selectively knocking out the Myd88 molecule in small intestinal epithelial cells. Background technique [0002] As we all know, dendritic cells (DCs) have long been considered as a powerful and diverse antigen-presenting cell in the human body. Mobilize the body's immune response to achieve physiological defense or pathological aggravation of damage. More recent studies have found that small intestinal epithelial cells not only serve as the main content of the intestinal mechanical barrier, but also have immune activity similar to the underlying function of antigens. [0003] The surface area of ​​the normal human small intestinal mucosa is 250 square meters, and there are 100 trillion bacteria in the intestinal lumen. Our previous study found that the immune cells in the intestinal submucosal may change the local and systemic immune s...

AI Technical Summary

Problems solved by technology

[0007] However, in what state the intestinal epithelium transitions from mediating tolerance to defense, and the exact molecular biological mechanism involved in this transition is still unclear.

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