Rapid separation and identification kit for streptococcus agalactiae and application for same

Abstract

The invention discloses a rapid separation and identification kit for streptococcus agalactiae. In the kit, the following reagents are included: sheep defibering blood plate, 3% H2O2, staphylococcus aureus strains, a specific PCR (polymerase chain reaction) solution, and positive control DNA (deoxyribonucleic acid). The rapid separation and identification kit for streptococcus agalactiae provided by the invention has the following beneficial effects that bacterial morphological observation, catalase test, CAMP (cyclic adenylic acid) reaction and PCR detection are used together, so that identification procedures can be simplified, the specificity is strong, and the sensitivity is increased by more than 15%; and moreover, the identification cost is low, the time consumption is short (only needing 3 days), and streptococcus agalactiae strains can be obtained by separation and accurately identified. The kit can be used for separation and identification for the samples of the milk samples of human or dairy cows, cervical mucus, vaginal swabs and the like.

Description

technical field [0001] The invention relates to the field of bacterial identification, in particular to a rapid isolation and identification kit for Streptococcus agalactiae and its application. Background technique [0002] There are more than 20 kinds of pathogenic bacteria that cause mastitis in cows. However, a large number of research results by scholars at home and abroad show that mastitis caused by Staphylococcus and Streptococcus infection is more common clinically, accounting for about 90%-95% of the total pathogenic bacteria. Among them, mastitis caused by Streptococcus agalactiae accounts for a very large proportion in the total incidence of mastitis. Li Hongsheng et al. (2004) isolated and identified 12 species and 1968 strains of pathogenic bacteria closely related to mastitis from 2858 milk samples of clinical type, latent mastitis and healthy milk samples, among which the total detection rate of Streptococcus agalactiae was the highest (38.47 %), and confir...

AI Technical Summary

Problems solved by technology

Using commercial biochemical detection reagents, even if the test is successful, it will take 7-10 days to obtain all the biochemical identification results. If the test is not smooth or the number of samples is increased, it will take longer, and some pathogenic bacteria may not be able to determine the results.

At present, the Mérieux API bacterial identification reagent strip (biochemical identification) commonly used in the world takes almost the same time as the identification of the present invention, but it also has the disadvantages of complicated operation, high identification cost, and sometimes uncertain identification results.

Simple PCR sometimes has interference from non-specific bands, resulting in inaccurate results

Although the accuracy of gene sequencing is high, there are defects such as high cost and long time, which affect its large-scale promotion and use in clinical practice.

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