Articles and methods for detecting target microorganisms

Abstract

The invention discloses a method for detecting target microorganisms. The method includes providing a culture device comprising a selective medium and a detection article comprising a first indicator system. The selective medium promotes the growth of indicator microorganisms. When an indicator microorganism is detected in a sample contacted with the medium, the detection article is contacted with the medium to detect the target microorganism.

Description

[0001] Cross References to Related Applications [0002] This patent application claims the benefit of US Provisional Patent Application No. 61 / 428,722, filed December 30, 2010, which is hereby incorporated by reference in its entirety. Background technique [0003] Testing samples of entire foods, beverages, and water for pathogenic microorganisms may be impractical both because of the high cost and because pathogenic microorganisms are rarely present in, for example, properly processed foods. Therefore, tests for the presence of indicator microorganisms are routinely used to test food and water for the possibility of contamination with human pathogens. The presence of one or more indicator microorganisms may indicate, for example, fecal contamination, and may indicate the possible presence of pathogenic microorganisms. [0004] Escherichia coli (coliform bacteria) represents an example of an indicator microorganism. Coliforms include several genera (eg, Citrobacter, Enter...

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Problems solved by technology

[0003] Testing samples of all food, beverages, and water for pathogenic microorganisms may be impractical due to high cost and because pathogenic microorganisms are rarely present in, for example, properly processed foods

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