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Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances

A technology of antibacterial activity and active substances, which is applied in the field of rapid identification of microorganisms producing antibacterial active substances and active substances, which can solve the problems of low efficiency and cumbersome steps, so as to improve efficiency, shorten experimental time, and save screening costs Effect

Inactive Publication Date: 2016-05-18
TAIZHOU VOCATIONAL & TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing technical steps are cumbersome and inefficient

Method used

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  • Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances
  • Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances
  • Method for rapidly identifying or screening microorganisms producing bacteriostatic active substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of method that the present invention proposes to quickly identify or screen the microorganisms that produce antibacterial active substances comprises the steps:

[0017] S1, prepare solid medium culture dish (LB medium or PDA medium etc.);

[0018] S2. Under aseptic conditions, inoculate the test microorganisms and indicator microorganisms by cross-streaking on the same petri dish;

[0019] S3. After inoculation, the culture dish is placed in an incubator for culture (culture conditions: 26-40° C., culture time 12-240 h);

[0020] S4. Determine whether the test microorganism produces an active substance that inhibits the indicator microorganism.

[0021] Such as figure 1 as shown, figure 1 It is a schematic diagram of Embodiment 1 of the present invention. Depend on figure 1 It can be seen that the test microorganism A does not produce the active substance that inhibits the indicator microorganism O, and the test microorganism A and the indicator microorgani...

Embodiment 2

[0023] A kind of method that the present invention proposes to quickly identify or screen the microorganisms that produce antibacterial active substances comprises the steps:

[0024] S1, prepare solid medium culture dish (LB medium or PDA medium etc.);

[0025] S2. Under aseptic conditions, inoculate the test microorganisms and indicator microorganisms by cross-streaking on the same petri dish;

[0026] S3. After inoculation, the culture dish is placed in an incubator for culture (culture conditions: 26-40° C., culture time 12-240 h);

[0027] S4. Determine whether the test microorganism produces an active substance that inhibits the indicator microorganism.

[0028] Such as figure 2 as shown, figure 2 It is a schematic diagram of Embodiment 2 of the present invention. Depend on figure 2 It can be seen that the test microorganism B produces an active substance that inhibits the indicator microorganism O, and only the test microorganism B grows at the intersection, whi...

Embodiment 3

[0030] A kind of method that the present invention proposes to quickly identify or screen the microorganisms that produce antibacterial active substances comprises the steps:

[0031] S1, prepare solid medium culture dish (LB medium or PDA medium etc.);

[0032] S2. Inoculate the test microorganism C (Bacillus amyloliquefaciens) and the indicator microorganism D (Escherichiacoli) cross-streaked on the same petri dish under aseptic conditions;

[0033] S3. Place the culture dish inoculated with microorganisms in an incubator for cultivation (cultivation conditions: 26-40° C., cultivation time 12-240 h);

[0034] S4. Determine whether the test microorganism produces an active substance that inhibits the indicator microorganism.

[0035] Such as image 3 as shown, image 3 It is the result photo of Example 3 of the present invention. Depend on image 3 It can be seen that the test microorganism C (Bacillus amyloliquefaciens) produced an active substance that inhibited the in...

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Abstract

The invention discloses a method for rapidly identifying or screening microorganisms producing bacteriostatic active substances. The method comprises steps as follows: a solid culture medium culture dish (an LB culture medium or a PDA culture medium and the like) is prepared; tested microorganisms and indicator microorganisms are inoculated to the same culture dish under the sterile condition with a cross-streak method; after inoculation, the culture dish is put in a culture box for inverted culture under the condition of 26-40 DEG C for 12-240 h; whether the tested microorganisms produce the active substances for inhibiting the microorganisms is determined. By means of the method, whether the tested microorganisms produce the active substances for inhibiting the indicator microorganisms can be rapidly identified or the microorganisms producing the active substances for inhibiting the indicator microorganisms can be rapidly screened, the steps are simple, and the efficiency is high.

Description

technical field [0001] The invention relates to the technical field of microbial experiments, in particular to a method for quickly identifying microorganisms producing antibacterial active substances or screening microorganisms producing active substances that have an inhibitory effect on indicator microorganisms. Background technique [0002] At present, the methods to identify or screen whether a microorganism produces antibacterial or antibacterial active substances are mainly agar diffusion method, disk diffusion method, drug susceptibility test (ie, inhibition zone method), growth rate method, confrontation method, cup and saucer method, etc. law etc. [0003] In the prior art, it is often operated separately first, that is, the fermentation product of the microorganism to be tested or the agar block with the microorganism is first obtained, and then the corresponding method is used for experimentation. If the microorganism to be tested produces an active substance tha...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 吴翰桂郝之奎杨美玲
Owner TAIZHOU VOCATIONAL & TECHN COLLEGE
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