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Inspection method for carrying out microbial drug sensitivity test by using drug sensitivity indicator

A technology of drug susceptibility test and inspection method, which is applied in the inspection field of microbial drug susceptibility test using drug susceptibility indicator, can solve the problems of long incubation time and inconspicuous judgment index of drug susceptibility test, achieve great application value and increase sensitivity and detection time, the effect of increasing sensitivity

Pending Publication Date: 2020-03-31
SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dilution method is usually carried out through microbial susceptibility plates, but there are certain deficiencies in such domestic products at present: (1) The incubation time of the susceptibility test is long; Judgment indicators are not obvious

Method used

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  • Inspection method for carrying out microbial drug sensitivity test by using drug sensitivity indicator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Drug susceptibility indicators are used with drug sensitivity slats:

[0030] 1) Weigh 5.0 mg of resazurin sodium salt, add purified water to make a 5 mg / ml solution, filter through a 0.22 μm sterile filter membrane and set aside.

[0031] 2) Add 72 μL of prepared 5 mg / ml resazurin sodium salt solution to the drug-susceptible inoculation culture medium.

[0032] 3) Use the Enterobacter drug-sensitivity plate (ME2) according to the instruction manual of the Enterobacter drug-sensitivity plate (ME2), and replace the original drug-sensitivity inoculation culture with the drug-sensitivity inoculation culture solution added with resazurin sodium salt solution in step 2). liquid for testing.

[0033] 4) After culturing for 20 hours, take out the Enterobacteriaceae susceptibility plate and use it separately: 1) Visually read the results, if the color of the indicator is red, pink or purple, it means the growth of microorganisms in the susceptibility test, and the antibacteria...

Embodiment 2

[0035] Drug susceptibility indicators are used with drug sensitivity slats:

[0036] 1) Weigh 5.0 mg of resazurin sodium salt, add purified water to make a 0.5 mg / ml solution, and filter it through a 0.22 μm sterile filter membrane for use.

[0037] 2) Use the Enterobacter susceptibility panel (ME2) according to the instructions of the Enterobacter susceptibility panel (ME2).

[0038] 3) After culturing for 20 hours, take out the Enterobacteriaceae drug susceptibility plates and use them separately, and add 10 μL of prepared 0.5 mg / ml resazurin sodium salt solution to each test well, and continue culturing for 60 min.

[0039] 4) Take out the enterobacteriaceae susceptibility plate and use them respectively: 1) Visually read the results, if the indicator color is red, pink or purple, it means that the microorganisms grow in the susceptibility test, and the antibacterial drugs have no effect. If the color of the indicator is blue, blue-purple or purple, it means that the micro...

Embodiment 3

[0041] 1) Weigh 5.0 mg of resazurin sodium salt, add purified water to prepare a 5 mg / ml solution, and filter it through a 0.22 μm sterile filter membrane before use.

[0042] 2) Weigh 5 mg each of penicillin and amikacin, and prepare a 5 mg / ml solution with sterile purified water.

[0043] 3) Take two groups of sterile test tubes, numbered A and B respectively, 12 in each group, add 4ml of sterile purified water to the first test tube of each group, and add 2ml of sterile purified water to each of the other tubes.

[0044] 4) Add 5mg / ml resazurin sodium salt solution to the test tubes of group A and group B, wherein the addition amount of 5mg / ml resazurin sodium salt solution in the 2ml test tube is 40μL, 5mg / ml in the 4ml test tube ml of resazurin sodium salt solution was added in an amount of 80 μL.

[0045] 5) Add 25.6 μL of prepared 5 mg / ml penicillin solution to the first test tube of group A test tube, and then doubly dilute to the subsequent 9 test tubes, which are nu...

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Abstract

The invention relates to an inspection method for a microbial drug sensitivity test by using a drug sensitivity indicator, which takes resazurin or soluble resazurin salt thereof as the drug sensitivity indicator and indicates a microbial drug sensitivity test result through a color and / or fluorescence signal. After the drug sensitivity indicator is combined with a drug sensitivity test, the sensitivity of the drug sensitivity test can be improved, the detection time of the drug sensitivity test can be shortened, and the drug sensitivity indicator has important clinical guidance significance and high application value.

Description

technical field [0001] The invention relates to a microbial drug susceptibility test, in particular to an inspection method for using a drug sensitive indicator to perform a microbial drug susceptibility test. Background technique [0002] In recent years, due to the widespread use of a wide variety of antibacterial drugs in clinical practice, bacterial drug resistance has become more and more common. Some bacteria can be resistant to 2-7 kinds of drugs at the same time. In addition to the continuous updating of clinical diagnosis and treatment methods, although the diagnosis and treatment effects have been improved, it has also brought more opportunities for cross-infection and lowered the immune status of the body. What is particularly noticeable is that the type of infection has undergone great changes and is still changing, which puts forward higher requirements for the detection of clinical microbial drug susceptibility. [0003] At present, the methods used in domesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/445
CPCC12Q1/18C12Q2304/22
Inventor 王龙沈奕萍
Owner SHANGHAI FOSUN LONG MARCH MEDICAL SCI CO LTD
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