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Urease-producing microbes and curing method for heavy metals in foundation

A technology for microorganisms and urease production, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete removal, high cost, generation of toxic sludge or other wastes, etc., and achieves good effect and cost. Low, short time effect

Active Publication Date: 2013-09-11
TSINGHUA UNIV
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Problems solved by technology

[0003] Traditional heavy metal treatment methods mainly include: chemical precipitation method, ion exchange method, evaporation concentration method, electrolysis method, activated carbon and silica gel adsorption method and membrane separation method, etc., but these methods have incomplete removal, high cost, generation of toxic sludge or Disadvantages such as other waste

Method used

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  • Urease-producing microbes and curing method for heavy metals in foundation
  • Urease-producing microbes and curing method for heavy metals in foundation
  • Urease-producing microbes and curing method for heavy metals in foundation

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Embodiment

[0032] Isolation and Screening of Urease-Producing Bacteria with the Ability to Immobilize Heavy Metals

[0033] 1. Sample collection

[0034] Sporosarcina pasteurii was obtained from the American type culture collection (American type culture collection), numbered ATCC11859, and Terrabacter tumescens was obtained from the General Microbiology Center of China Committee for the Collection of Microorganisms, numbered AS.1.2690.

[0035] 2. Isolation and screening of urease-producing strains

[0036] Urease-producing strains were isolated from soil samples collected from the Tsinghua University flower garden.

[0037] Bacteria have ureolytic enzymes, which can decompose urea to produce a large amount of ammonia, making the medium alkaline and red. In this experiment, this characteristic was used to enrich and culture the test samples under the conditions of 37°C and 5M high-concentration urea for 24 hours, and kill various microbial vegetative cells that could not tolerate and ...

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Abstract

Urease-producing microbes comprise sporosarcina antarctica UR53, sporosarcina koreensis UR47, sporosarcina sp. UR31 and bacillus lentus UR41 which have been preserved in China General Microbiology Center of Committee for Culture Collection of Microorganisms since 16th March, 2012, and respectively have the preservation numbers of CGMCC No.5916, CGMCC No. 5915, CGMCC No. 5913 and CGMCC No. 5914. A curing method for heavy metals in a foundation by using the urease-producing microbes comprises: placing the four microbes and microbes of sporosarcina pasteurii and terrabacter tumescens in a fermentation medium to carry out fermentation culture to obtain a bacterial liquid, adding a reaction solution urea into the bacterial liquid, then adding the bacterial liquid into a heavy-metal-containing solution to form a mixed solution so as to form a microbe-heavy-metal floccule in the mixed solution, and further to obtain water-insoluble heavy metal carbonates. The curing method for heavy metals in the foundation by using the urease-producing microbes has the advantages of short curing time, good effect, low cost and no secondary pollution to environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and solidifying heavy metals thereof, in particular to urease-producing microorganisms and a method for solidifying heavy metals in foundations. Background technique [0002] Heavy metal pollution means that due to human production and activities, the content of heavy metals in the environment is significantly higher than its background value. Due to the long-term retention and non-degradable characteristics of heavy metal ions, it has caused great damage to the ecological environment. With large-scale urban expansion and construction, many buildings are built near abandoned factories and garbage dumps, and the heavy metal pollution in the foundation is serious. Heavy metal elements can finally enter the organism through the food chain, destroy the normal metabolism of the organism, and seriously endanger human health. It has become an environmental problem that cannot be ignored. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 程晓辉李萌郭红仙
Owner TSINGHUA UNIV
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