Method for detecting fatty acid nutrition enhancers in food
A nutritional enhancer and fatty acid technology, applied in the field of detection, can solve the problems of unsuitable nutritional enhancer detection, unfavorable technical guarantee for safety evaluation, obsolete detection technical standards, etc., to meet the requirements of food supervision and inspection Need, low detection limit, easy operation
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[0037] Instruments used: Agilent 7890A / 5975C gas chromatography-mass spectrometer, equipped with 7683B autosampler, enhanced chemical workstation; column: HP-5MS capillary column (30m×0.25mm×0.32mm)
[0038] Materials used: 25% ammonia water, anhydrous methanol, ether, petroleum ether, n-hexane, water, α-linolenic acid methyl ester standard, γ-linolenic acid methyl ester standard product, linoleic acid methyl ester standard product, conjugated linoleic acid Oil methyl ester standard, arachidonic acid methyl ester standard, EPA methyl ester standard, DHA methyl ester standard, methanol solution of 4mol / L potassium hydroxide.
[0039] (1) Take infant milk powder purchased on the market as a sample, weigh 5.0000g of the sample and place it in a stoppered Erlenmeyer flask, dissolve it by ultrasonic shaking with 25mL of 65°C water, add 5 mL of 25% ammonia water for ultrasonic extraction for 15min, take it out and shake it to cool Bring to room temperature, add 10 mL of methanol and m...
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