Method for detecting fatty acid nutrition enhancers in food

A nutritional enhancer and fatty acid technology, applied in the field of detection, can solve the problems of unsuitable nutritional enhancer detection, unfavorable technical guarantee for safety evaluation, obsolete detection technical standards, etc., to meet the requirements of food supervision and inspection Need, low detection limit, easy operation

Inactive Publication Date: 2013-09-11
南通市产品质量监督检验所
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0007] These new nutritional fortifiers have been greatly developed in the preparation process, and have been widely used in production and use. However, the safety evaluation of the amount put into food as an additive has not been able to obtain favorable technical guarantees. The current national regulations The testing technical standards are relatively old, some are too old to keep up with the trend of the times, some are not suitable for the detection of nutritional fortifiers in food, and some are even blank in the national testing methods, there is no current effective standard, and many disadvantages are brought to the test. Regulating nutritionally fortified food production enterprises brings regulatory loopholes

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  • Method for detecting fatty acid nutrition enhancers in food
  • Method for detecting fatty acid nutrition enhancers in food
  • Method for detecting fatty acid nutrition enhancers in food

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Embodiment 1

[0037] Instruments used: Agilent 7890A / 5975C gas chromatography-mass spectrometer, equipped with 7683B autosampler, enhanced chemical workstation; column: HP-5MS capillary column (30m×0.25mm×0.32mm)

[0038] Materials used: 25% ammonia water, anhydrous methanol, ether, petroleum ether, n-hexane, water, α-linolenic acid methyl ester standard, γ-linolenic acid methyl ester standard product, linoleic acid methyl ester standard product, conjugated linoleic acid Oil methyl ester standard, arachidonic acid methyl ester standard, EPA methyl ester standard, DHA methyl ester standard, methanol solution of 4mol / L potassium hydroxide.

[0039] (1) Take infant milk powder purchased on the market as a sample, weigh 5.0000g of the sample and place it in a stoppered Erlenmeyer flask, dissolve it by ultrasonic shaking with 25mL of 65°C water, add 5 mL of 25% ammonia water for ultrasonic extraction for 15min, take it out and shake it to cool Bring to room temperature, add 10 mL of methanol and m...

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Abstract

The invention discloses a method for detecting fatty acid nutrition enhancers in food. According to the method, a GC-MS (gas chromatography-mass spectrometer) analysis method is adopted for detecting and analyzing the fatty acid nutrition enhancers in food, and chromatographic conditions and mass spectrum conditions are definite. The method for detecting the fatty acid nutrition enhancers in food comprises the following steps of: (1) extracting the fatty acid nutrition enhancers from food, and collecting an extracting solution; (2) detecting and analyzing the extracting solution by adopting the GC-MS method; (3) calculating a result and expressing; and (4) calculating tolerance. By applying the method for detecting the fatty acid nutrition enhancers in food, nutrition enhancers such as docosahexenoic acid (DHA), eicosapentaenoic acid (EPA), arachidonic acid (AA), linolenic acid, linoleic acid, conjugated linoleic acid and oleic acid and contents of the nutrition enhancers can be rapidly detected at the same time, and safety of the nutrition enhancers thrown into the food is evaluated; the method is simple and rapid in pretreatment process, easy to operate, and good in reproducibility, can meet the requirements of food supervising inspection and provides assistance for ordinary people to select real nutrient food.

Description

technical field [0001] The invention relates to a detection technology of a nutrition enhancer in food, in particular to a detection technology of a fatty acid nutrition enhancer in food, and belongs to the detection technology field. Background technique [0002] Food nutrition fortifiers refer to natural or synthetic food additives that are added to foods to enhance nutritional components, and fall within the scope of natural nutrients. In different foods, the distribution and content of nutrients are different. At the same time, nutrients may be lost during food cooking, processing, and storage. In order to maintain the original nutritional content of the food, or to supplement the lack of nutrients in the food, a certain amount of food nutritional fortifier is added to the food to improve its nutritional value. Such food is called nutritionally fortified food. Nutrients that need to be fortified in food include nutrients that are generally insufficiently supplied in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 丁红梅陈彬周鸣镝黄伟东陈刚邵彪
Owner 南通市产品质量监督检验所
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