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Method for preparing compound enzyme and probiotic preparation for feed by biotransformation of luffa stem and castor cake

A probiotic preparation and biotransformation technology, applied in animal feed, animal feed, applications, etc., can solve problems such as difficult digestion of monogastric animals, and achieve the effects of low-cost, high-efficiency biotransformation, and strong enzyme production capacity

Inactive Publication Date: 2013-09-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of loofah vines, 5kg of castor cake, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and mix them at 35°C After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0028] 2) Fully mix 20kg of the starter in step 1) with 600kg loofah vine, 300kg castor cake, 60kg wheat bran, 30kg apple pomace, and 800kg water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 44.67IU / g; total cellulase activity reaches 4.83IU / g; beta-glucosidase activity reaches 4.83IU / g; reached 11.25IU / g; endoglucanase activity reached 37.89IU / g; x...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh cooked tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of luffa vines, 5kg of castor cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 48 hours of acclimatization and cultivation, a leavening agent is formed;

[0032] 2) Fully mix 20kg of the starter in step 1) with 600kg loofah vine, 300kg castor cake, 60kg wheat bran, 30kg apple pomace, and 800kg water, and use solid-state fermentation with a material thickness of 15cm, naturally ventilated, at 40°C Ferment for 4 days to obtain the compound enzyme and probiotic preparation for feed.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 43.97IU / g; total cellulase activity reaches 4.91IU / g; beta-glucosidase activity reaches 4.91IU / g; reached 11.12IU / g; endoglucanase activity reached 37.36IU / g; xylanase activit...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu'er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of loofah vines, 5kg of castor cake, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water. After 36 hours of acclimatization and cultivation, a leavening agent is formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg loofah vine, 300kg castor cake, 60kg wheat bran, 30kg apple pomace, and 1000kg water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and fermented at 35°C After 5 days, a compound enzyme and probiotic preparation for feed was obtained.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 46.55IU / g; total cellulase activity reaches 5.05IU / g; beta-glucosidase activity reaches 5.05IU / g; reached 10.54IU / g; endoglucanase activity reached 34.44IU / g; xylanase activit...

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PUM

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Abstract

The invention discloses a method for preparing a compound enzyme and probiotic preparation for feed by biotransformation of luffa stem and castor cake. The method comprises the following steps of: fully mixing Pu'er ripe tea, shiitake mushroom log, agrocybe aegerita mushroom log, first luffa stem, first castor cake, first wheat bran, first apple pomace and first water which are fermented by piling, and performing acclimation culture so as to form a fermentation agent; and fully mixing the fermentation agent, second luffa stem, second castor cake, second wheat bran, second apple pomace and second water, and fermenting so as to obtain the compound enzyme and probiotic preparation for feed. In the method disclosed by the invention, a food-grade safe bacterial group with stable microecology is utilized for fermenting the luffa stem and the castor cake so as to prepare the compound enzyme and probiotic preparation for feed, which has various better performance indexes, and the low-cost and high-efficient biotransformation of the luffa stem and the castor cake can be realized. Simultaneously, the method disclosed by the invention has the advantages of easiness in growth of microbes, high enzyme activity, extensive fermentation process and easiness in industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting loofah vine and castor-bean cake by adopting micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖徐薇薇于骅林永华陈功诸文颖
Owner ZHEJIANG UNIV
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