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Application of beta-carotene in preparation of pig sperm cryoprotectant

A technology of cryoprotectant and carotene, which is applied in the biological field, can solve the problems of sperm cell membrane damage, pig sperm fertilization ability decline, etc., and achieve the effect of being easy to popularize, improving pig sperm viability, and maintaining fertilization vitality

Inactive Publication Date: 2013-10-02
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Relevant studies have shown that during the process of freezing and balancing semen, a large amount of reactive oxygen species (reactive oxygen species, ROS) is inevitably produced through respiratory metabolism, and the oxygen free radicals (O 2- 、H 2 o 2 and -OH) can oxidize the unsaturated fatty acids in the sperm cell membrane, causing damage to the sperm cell membrane, and finally leading to a sharp decline in the fertilization ability of pig sperm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g Dissolve in 100ml double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base fluid;

[0028] Accurately measure 80ml of conventional frozen base solution, add 20ml of fresh egg yolk and 1mg to 1.5mg of β-carotene, and mix well to prepare Ⅰ solution;

[0029] Take 97ml of solution I and add 3ml of glycerin to prepare 100ml of solution II, adjust the pH of solution II to 6.0-7.5, and the osmotic pressure to 286mosm / L, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.

Embodiment 2

[0031] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g Dissolve in 100ml double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base fluid;

[0032] Accurately measure 80ml of frozen base solution, add 20ml of fresh egg yolk and 1.5mg to 2.0mg of β-carotene, and mix well to prepare liquid I;

[0033] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, and the osmotic pressure to 286mosm / L, filter and sterilize, cool to room temperature, and put it in a refrigerator at 4°C for later use.

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Abstract

The invention discloses an application of beta-carotene in preparation of a pig sperm cryoprotectant. The pig sperm cryoprotectant is composed of a cryoprotective basic solution, beta-carotene, fresh egg yolks and glycerin. The additive amount of beta-carotene is 1 mg-2 mg in 100 mL cryoprotective basic solution, and the additive amount of fresh egg yolks is 20 mL in 100 mL cryoprotective basic solution. 97 mL cryoprotective basic solution with addition of beta-carotene and fresh egg yolks is taken, 3 ml glycerin is added, and then 100 ml pig sperm cryoprotectant is prepared. 100 mL cryoprotective basic solution comprises 1.1g glucose, 1.48 g citric acid, 2.42 g Tris, 0.06 g penicillin sodium and 0.1g streptomycin sulphate, and the rest is double distilled water. In the application, beta-carotene is firstly used in pig sperm cryopreservation, after thawing, the pig sperm motility rate, the plasma membrane intact rate and the acrosome intact rate are 51%, 53% and 52%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an antifreeze agent for animal semen, in particular to the application of beta-carotene in preparing a cryoprotectant for pig semen. Background technique [0002] Semen cryopreservation technology is developed along with artificial insemination technology, which is a major innovation in artificial insemination technology. The principle of semen cryopreservation technology is to use liquid nitrogen (-196°C), dry ice (-79°C) or other cold sources to properly process the sperm to completely stop sperm metabolism. Thaw artificial insemination and in vitro fertilization when used. Semen freezing can greatly increase the utilization rate of breeding males, reduce the amount of breeding males, save costs and is not limited by region and time. It is beneficial to the hybrid improvement of livestock breeds and is an effective technical means for livestock breeding and protection of endangered...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 李青旺胡晓辰江中良胡靓华
Owner NORTHWEST A & F UNIV
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