Application of beta-carotene in preparation of pig sperm cryoprotectant
A technology of cryoprotectant and carotene, which is applied in the biological field, can solve the problems of sperm cell membrane damage, pig sperm fertilization ability decline, etc., and achieve the effect of being easy to popularize, improving pig sperm viability, and maintaining fertilization vitality
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[0026] Example 1:
[0027] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g dissolved in 100ml of double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base liquid;
[0028] Accurately measure 80ml of conventional frozen base liquid, add 20ml of fresh egg yolk and 1mg~1.5mg of β-carotene, and mix well to prepare liquid I;
[0029] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, osmotic pressure of 286 mosm / L, filter sterilize, cool to room temperature, and put it in a refrigerator at 4°C for use.
Example Embodiment
[0030] Example 2:
[0031] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g dissolved in 100ml of double distilled water, adjust pH to 6.21, osmotic pressure 286mosm / L, Formulated into frozen base liquid;
[0032] Accurately measure 80ml of frozen base liquid, add 20ml of fresh egg yolk and 1.5mg~2.0mg of β-carotene, and mix it to prepare liquid I;
[0033] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, osmotic pressure of 286 mosm / L, filter sterilize, cool to room temperature, and put it in a refrigerator at 4°C for use.
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