Application of beta-carotene in preparation of pig sperm cryoprotectant
A technology of cryoprotectant and carotene, which is applied in the biological field, can solve the problems of sperm cell membrane damage, pig sperm fertilization ability decline, etc., and achieve the effect of being easy to popularize, improving pig sperm viability, and maintaining fertilization vitality
Inactive Publication Date: 2013-10-02
NORTHWEST A & F UNIV
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Abstract
The invention discloses an application of beta-carotene in preparation of a pig sperm cryoprotectant. The pig sperm cryoprotectant is composed of a cryoprotective basic solution, beta-carotene, fresh egg yolks and glycerin. The additive amount of beta-carotene is 1 mg-2 mg in 100 mL cryoprotective basic solution, and the additive amount of fresh egg yolks is 20 mL in 100 mL cryoprotective basic solution. 97 mL cryoprotective basic solution with addition of beta-carotene and fresh egg yolks is taken, 3 ml glycerin is added, and then 100 ml pig sperm cryoprotectant is prepared. 100 mL cryoprotective basic solution comprises 1.1g glucose, 1.48 g citric acid, 2.42 g Tris, 0.06 g penicillin sodium and 0.1g streptomycin sulphate, and the rest is double distilled water. In the application, beta-carotene is firstly used in pig sperm cryopreservation, after thawing, the pig sperm motility rate, the plasma membrane intact rate and the acrosome intact rate are 51%, 53% and 52%.
Application Domain
Dead animal preservation
Technology Topic
GlycerolCarotene +12
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