Application of beta-carotene in preparation of pig sperm cryoprotectant

A technology of cryoprotectant and carotene, which is applied in the biological field, can solve the problems of sperm cell membrane damage, pig sperm fertilization ability decline, etc., and achieve the effect of being easy to popularize, improving pig sperm viability, and maintaining fertilization vitality

Inactive Publication Date: 2013-10-02

NORTHWEST A & F UNIV

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AI-Extracted Technical Summary

Problems solved by technology

[0005] Relevant studies have shown that during the process of freezing and balancing semen, a large amount of reactive oxygen species (reactive oxygen species, ROS) is inevitably produced through respiratory metabolism, and the oxyge...

Abstract

The invention discloses an application of beta-carotene in preparation of a pig sperm cryoprotectant. The pig sperm cryoprotectant is composed of a cryoprotective basic solution, beta-carotene, fresh egg yolks and glycerin. The additive amount of beta-carotene is 1 mg-2 mg in 100 mL cryoprotective basic solution, and the additive amount of fresh egg yolks is 20 mL in 100 mL cryoprotective basic solution. 97 mL cryoprotective basic solution with addition of beta-carotene and fresh egg yolks is taken, 3 ml glycerin is added, and then 100 ml pig sperm cryoprotectant is prepared. 100 mL cryoprotective basic solution comprises 1.1g glucose, 1.48 g citric acid, 2.42 g Tris, 0.06 g penicillin sodium and 0.1g streptomycin sulphate, and the rest is double distilled water. In the application, beta-carotene is firstly used in pig sperm cryopreservation, after thawing, the pig sperm motility rate, the plasma membrane intact rate and the acrosome intact rate are 51%, 53% and 52%.

Application Domain

Dead animal preservation

Technology Topic

GlycerolCarotene +12

Examples

Experimental program(2)
Comparison scheme(1)
Effect test(1)

Example Embodiment

[0026] Example 1:

[0027] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g dissolved in 100ml of double distilled water, adjust pH to 6.21, osmotic pressure 286mosm/L, Formulated into frozen base liquid;

[0028] Accurately measure 80ml of conventional frozen base liquid, add 20ml of fresh egg yolk and 1mg~1.5mg of β-carotene, and mix well to prepare liquid I;

[0029] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, osmotic pressure of 286 mosm/L, filter sterilize, cool to room temperature, and put it in a refrigerator at 4°C for use.

Example Embodiment

[0030] Example 2:

[0031] Accurately measure glucose: 1.1g, citric acid: 1.48g, Tris: 2.42g, penicillin sodium: 0.06g, streptomycin sulfate: 0.1g dissolved in 100ml of double distilled water, adjust pH to 6.21, osmotic pressure 286mosm/L, Formulated into frozen base liquid;

[0032] Accurately measure 80ml of frozen base liquid, add 20ml of fresh egg yolk and 1.5mg~2.0mg of β-carotene, and mix it to prepare liquid I;

[0033] Take 97ml of liquid I and add 3ml of glycerin to prepare 100ml of liquid II, adjust the pH of liquid II to 6.0-7.5, osmotic pressure of 286 mosm/L, filter sterilize, cool to room temperature, and put it in a refrigerator at 4°C for use.

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Description & Claims & Application Information

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Application of beta-carotene in preparation of pig sperm cryoprotectant

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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