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Reproduction method of isoetes yunguiensis

A technology of Yunnan-Guizhou water leek and sterile water, which is applied in the field of plant propagation, can solve the problems that wild Yunnan-Guizhou water leek resources cannot be effectively protected, there is no large-scale reproduction technology of Yunnan-Guizhou water leek, and large-scale reproduction and production cannot be applied. Achieve the effect of realizing large-scale reproduction, meeting demand and reducing damage

Inactive Publication Date: 2015-06-17
HARBIN NORMAL UNIVERSITY
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  • Claims
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Problems solved by technology

At present, scientific research activities have a great demand for resources of Rhizoma yunguiensis, and there is also an urgent need for a large number of Rhizoma yunguiensis plants for wild protection and population restoration. However, there is no large-scale propagation technology of genus rhizomes, which leads to the inability to effectively obtain wild resources Protect
[0004] At present, among the 5 kinds of water leeks produced in China, only the sexual reproduction stage of Chinese water leek (Isoetes sinensis) can be cultivated, and the entire life cycle cannot be completed, nor can it be applied to large-scale reproduction and production.
Neither the small-scale cultivation nor the large-scale production of Yunnan-Guizhou water leek has been reported

Method used

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0018] A kind of breeding method of Yunnan-Guizhou water leek; Concrete steps are as follows:

[0019] (1) Production and sterilization of the culture box

[0020] Spread absorbent cotton with a thickness of 1cm evenly in a well-sealed transparent plastic box with a length of 30cm, a width of 20cm, and a height of 12cm; then spread a layer of filter paper on the absorbent cotton; moisten the absorbent cotton and filter paper with MS medium, and use just enough saturated with filter paper; the result is a culture box (such as figure 1 shown); put the culture box in a sterilizing pot, and sterilize by high-pressure steam at 121°C for 20 minutes.

[0021] (2) Disinfection of Yunnan-Guizhou Shuiyu megaspores

[0022] Take 600 large spores of Allium yunnanensis and put them in a 1.5ml centrifuge tube, drop them into sterile water, oscillate fully, centrif...

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Abstract

The invention discloses a reproduction method of isoetes yunguiensis. The method comprises the steps that spores of the isoetes yunguiensis are inoculated in a cultivation box for cultivating after disinfection, and then are transplanted and subjected to field planting management in a manual culture pond, and eventually mature sporophytes are obtained. With the adoption of the reproduction method of the isoetes yunguiensis, a small quantity of spore materials are utilized to reproduce a large quantity of sporophytes rapidly, juvenile sporophyte seedlings can be obtained in 70 days, mature sporophytes can be obtained in 210 days, and field planting survival rate can reach 50%. According to the reproduction method of the isoetes yunguiensis, large-scale reproduction of the isoetes yunguiensis is realized, and the defect that a resource of the isoetes yunguiensis can be obtained only from a wild environment in the past is overcome, so that the requirement of scientific research activities for the resource of the isoetes yunguiensis is met, the ex-situ conservation of the isoetes yunguiensis is possible, simultaneously, damage to wild resources and environment can be reduced, and the reproduction method of the isoetes yunguiensis has a wide application prospect.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a reproduction method of Yunnan-Guizhou water leek. Background technique [0002] Isoetes is a relict genus of Isoetaceae, which is an ancient aquatic or semi-aquatic fern, which originated between the late Devonian and early Carboniferous, and is of great importance in botanical research. value. The stems of the plants of the genus Lime are short and thick, and the leaves are long and narrow. There are sporangia at the base of the leaves, which produce megaspores and microspores. The two kinds of spores form female and male gametophytes respectively, and cooperate with each other to complete the sexual reproduction process. There are only about 200 species of water allium in the world, mainly in the northern hemisphere. There are only 5 species of genus Aquatica produced in my country, all of which are national first-class protected plants and are extremely endangered. They are ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G31/00
Inventor 刘保东李晓东
Owner HARBIN NORMAL UNIVERSITY
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