Reproduction method of isoetes yunguiensis
A technology of Yunnan-Guizhou water leek and sterile water, which is applied in the field of plant propagation, can solve the problems that wild Yunnan-Guizhou water leek resources cannot be effectively protected, there is no large-scale reproduction technology of Yunnan-Guizhou water leek, and large-scale reproduction and production cannot be applied. Achieve the effect of realizing large-scale reproduction, meeting demand and reducing damage
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[0017] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0018] A kind of breeding method of Yunnan-Guizhou water leek; Concrete steps are as follows:
[0019] (1) Production and sterilization of the culture box
[0020] Spread absorbent cotton with a thickness of 1cm evenly in a well-sealed transparent plastic box with a length of 30cm, a width of 20cm, and a height of 12cm; then spread a layer of filter paper on the absorbent cotton; moisten the absorbent cotton and filter paper with MS medium, and use just enough saturated with filter paper; the result is a culture box (such as figure 1 shown); put the culture box in a sterilizing pot, and sterilize by high-pressure steam at 121°C for 20 minutes.
[0021] (2) Disinfection of Yunnan-Guizhou Shuiyu megaspores
[0022] Take 600 large spores of Allium yunnanensis and put them in a 1.5ml centrifuge tube, drop them into sterile water, oscillate fully, centrif...
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