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Preparation method of potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and use

A technology of crude polysaccharide and fern, which is applied in the direction of respiratory diseases and drug combination, can solve the problem of poor therapeutic effect of non-specific cough, and achieve the effect of simple preparation process and strong antitussive effect

Inactive Publication Date: 2013-10-23
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Western medicine cough mostly acts on the central nervous system, sedation, constipation, nausea and vomiting and other side effects are likely to occur, and the clinical effect on non-specific cough with unclear etiology is poor

Method used

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  • Preparation method of potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and use
  • Preparation method of potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take the obtained Junima powder after pulverization, add the concentration to 95% ethanol solution, under the condition that the solid-to-liquid ratio is 1:20 (g / mL), the extraction time is 45min, reflux extraction twice, filter, Get the dregs. Boil the dregs with 10 times the amount of distilled water or deionized water to extract 3 times, filter, cool to room temperature, decompress the filtrate to 1 / 5 of the original volume, add 4 times the amount of 95% ethanol to the concentrated solution at 4 °C Stand still for 24 - 72h, and centrifuge to obtain crude polysaccharide precipitate. The precipitate was washed several times with ethanol and acetone, respectively, and freeze-dried to obtain 10.1% Bracken japonicus polysaccharide extract.

Embodiment 2

[0025] Take the obtained Junima powder after pulverization, add it into the ethanol solution with a concentration of 95%, under the condition that the solid-to-liquid ratio is 1:20 (g / mL), the extraction time is 60min, reflux extraction 3 times, filter, Get the dregs. Boil the dregs with 20 times the amount of distilled water or deionized water to extract 3 times, filter, cool to room temperature, decompress the filtrate to 1 / 5 of the original volume, add 4 times the amount of 95% ethanol to the concentrated solution at 4 °C Stand still for 24 - 72h, and centrifuge to obtain crude polysaccharide precipitate. The precipitate was washed several times with ethanol and acetone respectively, and freeze-dried to obtain 11.4% Bracken japonicus polysaccharide extract.

[0026] Antitussive test of fern polysaccharide:

[0027] (1) Ammonia water induced cough method:

[0028] Sixty Kunming mice, half male and half female, fasted for 12 hours, were placed in 500 mL beakers, a cotton b...

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Abstract

A preparation method of a potentilla anserina L. root crude polysaccharide with potentilla anserina L. root as raw material and a use are provided. The method comprises the steps: taking the potentilla anserina L. root medicinal material, and crushing to obtain a potentilla anserina L. root powder; taking the potentilla anserina L. root powder; adding 5-20 times the amount of 60-95% alcohol for immersion or refluxing extraction for 0.5-2 h, repeating 2-3 times, and filtering to remove the alcohol solution; adding water with at least 4 times the weight of the potentilla anserina L. root powder into the potentilla anserina L. root dregs, heating for extraction, after finishing extraction, cooling to the room temperature, filtering to obtain a filtrate, extracting, and repeating 2-3 times; merging the filtrates; carrying out reduced pressure concentration of the merged filtrate until the volume is 1 / 6-1 / 3 that of the original merged filtrate; adding 4 times the amount of 95% ethanol into the concentrated solution, then standing for 24-72 h at the temperature of 4 DEG C, and centrifuging to obtain a crude polysaccharide precipitate; and respectively washing the precipitate with ethanol and acetone for a plurality of times, and carrying out freeze drying to obtain a potentilla anserina L. root polysaccharide extract. The prepared polysaccharide has the antitussive use.

Description

technical field [0001] The invention relates to the preparation technology and medicinal use of Tibetan medicine. Background technique [0002] Potentia genus Potentilla anserina The enlarged root tuber of L., also known as Juma Potentilla, Qujian Potentilla, Ginseng Fruit, Prolonged Life Grass, Lotus Cauliflower, is called "Zhuo Laosha Monk" and "Ta Ma" in Tibetan. It is a commonly used Tibetan medicine. Widely distributed in the western region of my country, especially in the Qinghai-Tibet Plateau, it has been frequently used in Tibetan medicine for more than a thousand years. Juma is flat and sweet, and has the effects of strengthening the spleen and stomach, promoting body fluid and quenching thirst, astringent and hemostasis, and nourishing qi and blood. It is suitable for diarrhea, spleen deficiency, anemia after illness, bleeding hemorrhoids, malnutrition, spleen and kidney yang deficiency and other diseases. Bracken contains carbohydrates, proteins, fatty acids (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P11/14
Inventor 郭涛张飞李永辉魏俊青王雅陈晓前
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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