Asarum total lignan extract, its extraction method and its use in the preparation of medicines for alleviating or suppressing cough

Abstract

The invention discloses an asarum total lignan extract and a preparation method and application thereof, belonging to the technical field of medicines. The preparation method comprises the following steps: extracting the root and / or tuber of asarum with a polar solvent and then carrying out concentration and drying so as to obtain an asarum total extract; and dissolving the asarum total extract with water, injecting the dissolved asarum total extract into a macroporous adsorption resin, carrying out gradient elution by using an aqueous ethanol solution, collecting eluate, recovering the solvent and carrying out drying so as to obtain the asarum total lignan extract with a content of 60 to 80 wt%, wherein yield of the asarum total lignan extract is 8.4 wt%. Through a great number of pharmacodynamic tests, it is proved for the first time that the asarum total lignan extract has substantial cough-inhibiting and cough reflex sensitivity-lowering effects which are close to the effects of codeine, and it is found out that the asarum total lignan extract has effects on inhibiting airway inflammations, reducing coughing frequencies and prolonging the incubation period of cough, exerts good prevention and treatment effects on a variety of coughs and can be used for preparation of drugs used for treating diseases related to cough. The asarum total lignan extract has the advantages of definite activity, usage of original plants as experimental materials and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicines, and more specifically relates to an extract of total lignans from Herba Asarum and its extraction method and its use in preparing medicines for alleviating or suppressing cough. Background technique [0002] Due to many factors such as work pressure and air pollution, the incidence of human respiratory diseases remains high, and cough is a common and frequently-occurring disease of the respiratory system, and it is a common complaint in respiratory clinics. Cough itself is a defensive reflex activity produced when the respiratory system is stimulated. It is a beneficial action and is sometimes seen in healthy people. In general, mild and infrequent coughs can be relieved naturally without the use of cough medicine. In addition, the impact of cough on human health is also dialectical, and it is also a pathological response. Long-term frequent or too intense cough will not only increase the suff...

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Problems solved by technology

Our previous research found that: Asarum volatile oil has obvious antitussive effect on the acute cough guinea pig model stimulated by citric acid, but it has no obvious antitussive effect on the cough hypersensitivity guinea pig model exposed to cigarettes. Su is good, and large doses have certain side effects

In addition, there are few research reports on the non-volatile components of Asarum. Bell amide Ⅰ, kaempferol-3-O-glucoside, etc. (Cai Shaoqing. Research on the non-volatile chemical components of Asarum. Beijing Medical University Journal. 1996,28(3):228-230; Shuai Lv. Chemical constituents of the underground part (Ⅱ). Journal of Shenyang Pharmaceutical University. 2010, 27(9): 707-710; Wei Qingchun. Isolation and identification of chemical constituents of Hancheng Asarum. Shi Zhen Guo Yi Guo Yao. 2010, 21 (3) :676-677)

At present, there is no report on the preparation method and content determination of total lignans from Asarum, let alone the research report on the cough-relieving activity of total lignans from Asarum

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