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ShRNA of human RUNX2 gene, and recombinant interference vector and application thereof

A technology of gene and gene expression, applied in the field of recombinant interference carrier and application, to achieve the effect of reducing the ball forming ability

Inactive Publication Date: 2015-02-18
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

However, there is no report on the relationship between RUNX2 and the invasion and metastasis of gastric cancer.

Method used

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  • ShRNA of human RUNX2 gene, and recombinant interference vector and application thereof
  • ShRNA of human RUNX2 gene, and recombinant interference vector and application thereof
  • ShRNA of human RUNX2 gene, and recombinant interference vector and application thereof

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Embodiment Construction

[0020] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0021] 1. Acquisition of specific interference sequences targeting human RUNX2 gene

[0022] Design the shRNA sequence according to the human RUNX2 gene sequence published by NCBI (Genbank: NM_001024630). The specific design method is to use the RNAi online provided by the website of Invitrogen, USA (the website is http: / / rnaidesigner.invitrogen.com / rnaiexpress / sort.do) Design software, screen the target sequence of interference according to the human RUNX2 gene sequence, and obtain the target sequence containing 21 bases after comparison and screening, the specific sequen...

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Abstract

The invention discloses an shRNA of a human RUNX2 gene, and a recombinant interference vector and an application thereof. The nucleotide sequence of the shRNA is represented by SEQ ID NO.2 from the 5th position to the 52th position, and the sequence can interfere the expression of the RUNX2 gene and reduce the cell balling capability, the cell cloning formation capability and the cell invasion capability. The shRNA can reduce the expression of stem transcription factors comprising a transcription factor Sox2, a transcription factor Oct4 and a transcription factor Bmi1 after interfering the expression of the RUNX2 gene, can delay the invasion and transfer of stomach cancer cells, and can be used in stomach cancer treatment medicines.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to shRNA of human RUNX2 gene, and also relates to a recombinant interference vector expressing the shRNA and its application. Background technique [0002] Globally, gastric cancer is the third leading cause of cancer death. In Asia, gastric cancer is the malignant tumor of the digestive tract with the highest morbidity and mortality. Although there are many early detection and diagnosis methods including digestive endoscopy, the early diagnosis rate of gastric cancer is still low, and the proportion of gastric cancer patients with distant spread and metastasis at the first diagnosis is as high as 60%. At present, the methods used in the treatment of gastric cancer are mainly surgical resection combined with a variety of chemotherapeutic drugs. In recent years, with the innovation of diagnostic methods and the improvement of treatment methods, the therapeutic effect of gastr...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/867A61K48/00A61P35/00
Inventor 郭政军卞修武杨浪任勇刘强姬成东崔巍王强崔有宏
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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