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Zizhi liquid submerged fermentation mycelia homogeneous polysaccharide and its preparation method and application

A technology of submerged liquid fermentation and mycelium, applied in allergic diseases, antineoplastic drugs, drug combinations, etc., can solve the problems of inability to investigate the mechanism of antitumor action, unclear active ingredients, and suboptimal dosage, etc., to achieve active The ingredients are single and clear, the dosage is excellent, and the dosage is more effective

Active Publication Date: 2017-12-05
JIANGSU KANION PHARMA CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the above patent applications and articles involved the study of homogeneous polysaccharides with anti-tumor activity, and the crude polysaccharides had deficiencies such as low polysaccharide content, poor dosage, unclear active ingredients, and no way to investigate the mechanism of anti-tumor action.

Method used

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  • Zizhi liquid submerged fermentation mycelia homogeneous polysaccharide and its preparation method and application
  • Zizhi liquid submerged fermentation mycelia homogeneous polysaccharide and its preparation method and application
  • Zizhi liquid submerged fermentation mycelia homogeneous polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of the homogeneous polysaccharide GS-A-1 from Zizhi liquid submerged fermentation mycelium:

[0040] 1) Preparation of crude polysaccharide:

[0041]Zizhi liquid submerged fermentation mycelium (the Zizhi liquid submerged fermentation liquid is obtained by the method described in Chinese patent 200710045369.4 "A Zizhi fermentation method and prepared Zizhi mycelia"). The specific method is as follows: Zizhi purchased from Shandong Ganoderma sinense was isolated from G. sinense, inoculated in a sterile room on a plate medium sterilized at 121°C for 30 minutes, and cultured at 28°C. After 4-6 days, inoculate the strains in the shaker flask seed medium that was sterilized at 121°C for 30 minutes in a sterile room, and cultivate them with shaking at 28±1°C for 5-7 days on a shaker, and select better strains for fermentation Cultivate in a tank for 5-6 days to obtain liquid fermented mycelia of Zizhi lucidum.

[0042] Extract the mycelium with water ...

Embodiment 2

[0048] Example 2: Determination of the polysaccharide content of the homogeneous polysaccharide GS-A-1 of the Zizhi liquid submerged fermentation mycelia by the phenol-sulfuric acid method

[0049] Specific steps are as follows:

[0050] Preparation of standard product: Accurately weigh 25 mg of dry glucose, dissolve it in water and set the volume to 100 ml, and make a 250 ug / ml standard product solution, take 0.05, 0.1, 0.15, 0.2, 0.25, and 0.3 mL in test tubes, add Make up to 0.3mL with ionic water, add 0.7mL of 5% phenol and shake well, quickly add 4mL of concentrated sulfuric acid, bathe in 50°C water for 40min, cool to room temperature, and use it as a reference substance.

[0051] Preparation of blank reference substance: Take 0.6ml of deionized water, add 1.4ml of 5% phenol, 8ml of concentrated sulfuric acid, heat in a water bath at 50°C for 40min, cool to room temperature, and use it as a blank solution.

[0052] Standard curve drawing: Measure the absorbance value of...

Embodiment 3

[0055] Example 3: Determination of the molecular weight of the polysaccharide in the homogeneous polysaccharide GS-A-1 of the Zizhi liquid submerged fermentation mycelium of the present invention with the dextran standard product Dextron series

[0056] The specific method is as follows:

[0057] Chromatographic conditions: TSK-GEL GMPWxl chromatographic column; mobile phase: water; flow rate: 0.3mL / min; column temperature: 35°C; detector: differential detector.

[0058] The standard Dextron and GS-A-1 samples were dissolved in water and injected.

[0059] Determine the retention time of dextran Dextron series and GS-A-1, and use the retention time of Dextron-the molecular weight logarithmic value of Dextron series to make a standard curve. According to the retention time of GS-A-1 and the standard curve, it can be known that GS-A- 1 The peak molecular weight is 21190 Daltons.

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Abstract

The invention discloses a preparation method of a homogeneous polysaccharide with a new structure in the liquid submerged fermentation mycelium of Zizhi, and its application in the preparation of antitumor drugs and immunomodulatory drugs. It is characterized in that, the total polysaccharide content of the homogeneous polysaccharide is more than 95% by weight, and the molecular weight of the homogeneous polysaccharide is 10,000-30,000 Daltons, preferably 21,190 Daltons, and the polysaccharide shows high efficiency and low performance in anti-tumor applications. Toxins improve immunity. The liquid submerged fermentation mycelium homogeneous polysaccharide provided by the method has higher polysaccharide content, higher purity and better dosage, and its preparation process is also suitable for industrial production.

Description

technical field [0001] The present invention relates to the homogeneous polysaccharide GS-A-1 of Zizhi liquid submerged fermentation mycelium, its preparation method and its use in the preparation of antitumor drugs and immunomodulators. Background technique [0002] Tumor is one of the most common and serious diseases that directly threaten human life in today's world, so how to effectively prevent and treat tumor has become a hot spot in the medical field. While prolonging the life of cancer patients, traditional surgery, radiotherapy and chemotherapy will produce side effects such as damage to internal organs and suppression of immune function, which will significantly reduce the quality of life of patients. At present, fungal polysaccharides are often used in the adjuvant therapy of tumors because of their advantages of less toxic side effects, high safety, and good tumor inhibition effect. [0003] Edible and medicinal fungi have various functions such as anti-tumor an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00A61P37/04
Inventor 杨义芳肖伟牛莉鑫胡晓魏丹丹王振中赵祎武宋亚玲
Owner JIANGSU KANION PHARMA CO LTD
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