Preparation method for composite probiotic-fermented jam
A technology of compounding probiotics and probiotics, applied in the field of bioengineering, can solve problems that have not yet occurred, and achieve the effects of delaying aging, enhancing immunity, and inhibiting the reproduction of cancer cells
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Embodiment 1
[0035] The 4 strains of probiotics used in the present invention are all purchased from China Industrial Microorganism Culture Collection Center, which are respectively: Lactobacillus acidophilus CICC6082, Lactobacillus paracasei CICC6235, Streptococcus thermophilus CICC6218, Lactococcus lactis CICC23609. Abbreviated as: A bacteria, B bacteria, C bacteria, D bacteria.
[0036] 1. Selection of probiotics and preparation of medium
[0037] Bacteria A - Lactobacillus acidophilus CICC6082 ( Lactobacillus acidophilus ), medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 2.0g, MgSO 4 .7H 2 O 0.2g, MnSO 4 .H 2 O 0.05g, CaCO 3 20.0g, 1.0L distilled water, pH6.8.
[0038] B bacteria - Lactobacillus paracasei CICC6235 ( Lactobacillus paracasei ), medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g ...
Embodiment 2
[0061] 1. Selection of probiotics and preparation of medium
[0062] Bacteria A - Lactobacillus acidophilus CICC6082 ( Lactobacillus acidophilus ), medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 2.0g, MgSO 4 .7H 2 O 0.2g, MnSO 4 .H 2 O 0.05g, CaCO 3 20.0g, 1.0L distilled water, pH6.8.
[0063] B bacteria - Lactobacillus paracasei CICC6235 ( Lactobacillus paracasei ), medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0g Tween 80, K 2 HPO 4 2.0g, MgSO 4 .7H 2 O 0.2g, MnSO 4 .H 2 O 0.05g, CaCO 3 20.0g, 1.0L distilled water, pH6.8.
[0064] Bacteria C——Streptococcus thermophilus CICC6218 ( Streptococcus thermophilus ), the culture medium is: tryptone 15g / L, soybean protein 5g / L, NaCl 5g / L, sterilized by moist heat at 121°C for 15min, and cooled to about 50°C. Under sterile condi...
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