Streptomyces griseochromogenes YN-6 and application thereof to club root control
A technology of Streptomyces chromogenes, YN-6, applied in the application, bacteria, fungicides and other directions, can solve the problems of clubroot disease difficulty, long cycle, susceptible to disease, etc., to achieve strong adaptability and colonization ability, effect Stable and good control effect
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Embodiment 1
[0023] The preparation of embodiment 1 streptomyces griseus chromogenic YN-6 culture fluid
[0024] Streptomyces griseochromogenes YN-6 (CCTCC No: M2013187) isolated from the fungus-carrying soil of cruciferous crops infected with clubroot disease was activated on Gao’s No. 1 liquid medium plate, and then punched Take 2 bacteria cakes with a diameter of 5mm and put them into a 250mL Erlenmeyer flask containing 150mL Gaoshi No. 1 liquid medium. Under the dark condition of 28°C and 180r / min, shake and cultivate in a shaker for 6 days, and use sterilized gauze (4 Layer) was filtered to obtain Streptomyces YN-6 culture fluid.
Embodiment 2
[0025] Example 2 Evaluate the control effect of strain YN-6 on clubroot of cruciferous crops by inhibiting the germination of clubroot pathogen dormant spores and the greenhouse pot test
[0026] The indicator strains used in this example are Colletotrichum geloeosporiolides, Ustiloginoidea virens, Phytophthorac apsici, Colletotrichum spp., Pyricularia spp. oryzae).
[0027] 1 Inhibition of germination of dormant spores of clubroot pathogen
[0028] Add 2mL, 2mL, and 20mL of culture fluid of strain YN-6, suspension of dormant spores of clubroot pathogen, and cabbage root exudate into sterilized Erlenmeyer flasks, and use sterile water instead of culture fluid of antagonistic bacteria as a control. The value was 6.3, cultured in the dark at 24°C, and after 5 days, stained with lichen red for 3 minutes to check the germination of dormant spores under a microscope. Repeated 3 times, the results showed that the inhibition rate of germination of dormant spores of clubroot was 61.7...
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