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Streptomyces griseochromogenes YN-6 and application thereof to club root control

A technology of Streptomyces chromogenes, YN-6, applied in the application, bacteria, fungicides and other directions, can solve the problems of clubroot disease difficulty, long cycle, susceptible to disease, etc., to achieve strong adaptability and colonization ability, effect Stable and good control effect

Active Publication Date: 2014-12-10
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it takes a long time and a long cycle to cultivate disease-resistant varieties, and clubroot bacteria have physiological race differentiation, and the resistant varieties will become susceptible after several years of application. Therefore, it is very difficult to completely control clubroot through disease-resistant varieties.
[0005] Agricultural control methods such as soaking seeds in warm soup, adding slaked lime or plant ash, etc. These treatment methods also have certain limitations. For example, applying slaked lime changes the acidity and alkalinity of the soil, but long-term use will destroy the soil structure, so it is not suitable for long-term use.

Method used

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  • Streptomyces griseochromogenes YN-6 and application thereof to club root control
  • Streptomyces griseochromogenes YN-6 and application thereof to club root control
  • Streptomyces griseochromogenes YN-6 and application thereof to club root control

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The preparation of embodiment 1 streptomyces griseus chromogenic YN-6 culture fluid

[0024] Streptomyces griseochromogenes YN-6 (CCTCC No: M2013187) isolated from the fungus-carrying soil of cruciferous crops infected with clubroot disease was activated on Gao’s No. 1 liquid medium plate, and then punched Take 2 bacteria cakes with a diameter of 5mm and put them into a 250mL Erlenmeyer flask containing 150mL Gaoshi No. 1 liquid medium. Under the dark condition of 28°C and 180r / min, shake and cultivate in a shaker for 6 days, and use sterilized gauze (4 Layer) was filtered to obtain Streptomyces YN-6 culture fluid.

Embodiment 2

[0025] Example 2 Evaluate the control effect of strain YN-6 on clubroot of cruciferous crops by inhibiting the germination of clubroot pathogen dormant spores and the greenhouse pot test

[0026] The indicator strains used in this example are Colletotrichum geloeosporiolides, Ustiloginoidea virens, Phytophthorac apsici, Colletotrichum spp., Pyricularia spp. oryzae).

[0027] 1 Inhibition of germination of dormant spores of clubroot pathogen

[0028] Add 2mL, 2mL, and 20mL of culture fluid of strain YN-6, suspension of dormant spores of clubroot pathogen, and cabbage root exudate into sterilized Erlenmeyer flasks, and use sterile water instead of culture fluid of antagonistic bacteria as a control. The value was 6.3, cultured in the dark at 24°C, and after 5 days, stained with lichen red for 3 minutes to check the germination of dormant spores under a microscope. Repeated 3 times, the results showed that the inhibition rate of germination of dormant spores of clubroot was 61.7...

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Abstract

The invention provides a Streptomyces griseochromogenes YN-6 separated from germ-carrying soil of cruciferae crops infected with club root, and the CCTCC No is M2013187. The strain YN-6 has good control effect on the club root of the cruciferae crops through verification of club root germ resting spore germination inhabitation and greenhouse pot experiments. The strain is high in adaptability and colonization capacity under field natural conditions, not prone to antagonistic reaction with complicated microorganism species in field soil, the disease control effect is stable, defects of chemical control, disease-resistant variety and agricultural control methods are effectively overcome, and the strain is an ideal anti-biological inoculant applicable to field club root control.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a strain of Streptomyces griseochromogenes YN-6 and its application in preventing and treating clubroot. Background technique [0002] Cruciferous crop clubroot is a worldwide soil-borne plant disease caused by Plasmodiophora brassicae infection. Plasmodium brassicae is an obligate parasite. It overwinters with its dormant spores in the soil with diseased residues and undecomposed manure. It has a strong survival ability and can generally survive for at least 8 years. It can survive for more than 15 years under suitable conditions. . At present, the main control measures for clubroot of cruciferous crops include chemical control, utilization of disease-resistant varieties, agricultural control and biological control. [0003] Chemical control is a relatively common method of prevention and control. Carbendazim, pentachloronitrobenzene, chlorothalonil, fluazide (fluazinam), and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G7/06C12R1/465
Inventor 刘二明孙良菲任佐华刘敏捷刘双清
Owner HUNAN AGRICULTURAL UNIV
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