Antitumor compounds derived from G. nujiangnsis, and preparation method and application thereof
A compound, anti-tumor technology, applied in the field of natural compounds
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[0018] 1. Preparation of anti-tumor compounds
[0019] a) The Nujiang Garcinia (collected from Nujiang Prefecture, Yunnan Province in August 2010, the plant sample was identified by Professor Zhou Yuanchuan of Yunnan College of Traditional Chinese Medicine, and the sample was kept in the Chinese Medicine Innovation Laboratory of Shanghai University of Traditional Chinese Medicine, marked as GN0001) and extracted with acetone , The volume of acetone used for each soaking is 5 times the mass of Garcinia Nujiangensis to be extracted. Each soaking for one week, a total of 3 soaking extractions; the acetone soaking extracts are combined and concentrated under reduced pressure until there is no acetone smell; The concentrated solution was extracted with dichloromethane 4 times, and the dichloromethane extract was concentrated under reduced pressure to obtain the extract;
[0020] b) The obtained extract is separated by silica gel column chromatography: gradient elution is carried out wit...
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