Application of protein fingerprinting model of Acidophilus avenatum subsp. watermelon

A oat acid phage and fingerprint technology, applied in the field of microbial detection, can solve the problem of no protein fingerprint model detection method, etc., and achieve the effect of strong specificity

Inactive Publication Date: 2016-01-06
SHENZHEN AUDAQUE DATA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no protein fingerprint model for detection or identification of A. avenatum subsp. watermelon, and there is no detection method based on the protein fingerprint model

Method used

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  • Application of protein fingerprinting model of Acidophilus avenatum subsp. watermelon
  • Application of protein fingerprinting model of Acidophilus avenatum subsp. watermelon
  • Application of protein fingerprinting model of Acidophilus avenatum subsp. watermelon

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Embodiment

[0030] 1. Materials

[0031] 1. Tested strains

[0032] In this example, 22 strains of A. avenatum subsp. watermelon and 17 strains of A. avenatum subsp. watermelon similar species were used for testing. Among them, 22 strains of A. avenatum subspecies watermelon include the numbers 470144 (ZZ-1), 470141 (SY-1), 470142 (SY-3), 470150 (LS-2) provided by the Seed Pathology Laboratory of China Agricultural University. ), 470138 (LS-3), 470140 (LS-10), 470131 (XJ-1), 470133 (XJ-4), 470139 (LS-5), 470136 (XJ-6), 470152 (BJ-A) The 10 strains provided by the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 470145 (pslb-25) and No. 470149 (FC512), 470126 (FC376), 470125 (FC374) provided by USDA / ARS / FDWSRU , 470148 (FC455), 470123 (FC248), 470122 (FC247 T , ATCC29625), 470121 (FC183), 470127 (FC380), 470130 (FC520), 470151 (pslb91) of 10 strains. The 17 other tested strains include 4 Acidovoraxavenaesubsp.avenae strains provided by USDA / ARS / FDWSRU with n...

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Abstract

This application discloses a protein fingerprint model of A. avenae subsp. watermelon. The model is drawn from the protein mass-to-charge ratio and peak intensity coefficient of A. avenae subsp. watermelon. , 5866.4, 6554.1, 7466.1, 7948.0, 8384.5, 9304.2, 9504.3, 9920.0, 10272.8, 10840.7, 12530.5, 12896.5. The application also discloses a matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection method based on the model. This method can qualitatively detect whether the sample contains Acidophage avenatum subsp. watermelon. The model of the present application has a specific recognition function, and can be used for the detection of commercial Acidophilus avenatum watermelon subspecies, and is especially suitable for the rapid and high-throughput detection of port inspection and quarantine departments.

Description

technical field [0001] The invention relates to the field of microbial detection, in particular to a protein fingerprint model of A. avenae subsp. watermelon and the application of the model in the detection and identification of A. avenae subsp. watermelon. Background technique [0002] Bacterial fruit spot of watermelon is a worldwide disease that seriously damages Cucurbitaceae crops, especially watermelon and muskmelon. The disease pathogen was first named by Schaad et al. as Pseudomonas pseudoalcaligens subsp.citrulli. In 1992, Willems et al. renamed it Acidovorax avenae subsp. citrulli (Aac.) based on the hybridization results of rRNA-RNA and DNA-DNA molecules. At present, the disease occurs in more than 10 states in the southeastern United States every year. Although the area of ​​occurrence is less than 5% in some years, the loss of diseased fields can be as high as 90% to 100%. Because the pathogen can be carried by seeds, it has caused a huge impact and threat to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
Inventor 冯建军章桂明李健强郑耘李一农娄定风
Owner SHENZHEN AUDAQUE DATA TECH
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